cell type: Endothelial cell expressed blood antigen A
Treatment protocol
EA.hy926/A cells were stimulated IFNγ in the presence or absence of anti-A antibody for 48hrs.
Growth protocol
Blood group A or B antigen-expressing EA.hy926 cells (HLA-A; *24:02, *25:01, HLA-B; *15:01, *18:01, HLA-Cw; *03:03, *12:03, HLA-DRB1; *11:02, *13:42, HLA-DQB1; n/d, HLA-DPB1; n/d), the human endothelial-like immortalized cell line derived from the fusion of human umbilical vein endothelial cells (HUVEC) with the lung carcinoma cell line A549, were maintained in DMEM supplemented with 10% FBS (Hyclone, Logan, UT).
Extracted molecule
total RNA
Extraction protocol
Total RNA was purified from cultured cells using the Qiazol and miRNeasy mini kits (Qiagen).
Label
biotin
Label protocol
Affymetrix
Hybridization protocol
Affymetrix
Scan protocol
Affymetrix
Description
Gene expression data from endothelial cells
Data processing
We employed ClariomTM S Assay based on affimatrix (Filgen Inc., Nagoya, Japan).
