|
| Status |
Public on Feb 28, 2019 |
| Title |
HeyA8-MDR miR204, rep2 |
| Sample type |
RNA |
| |
|
| Source name |
HeyA8-MDR miR204
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: HeyA8-MDR
|
| Treatment protocol |
HeyA8-MDR cells were treated with miR-204-5p-inhibitor.
|
| Growth protocol |
HeyA8-MDR cells were maintained in RPMI 1640 medium supplemented with 15% FBS, 300 ng/ml paclitaxel, and 0.1% gentamicin sulfate.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Trizol extraction of total RNA was performed according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
The fragmented cDNA was labeled by terminal deoxynucleotidyl transferase using the GeneChip WT PLUS Reagent Kit ( Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 5 micrograms of biotin-labeled sense-strand cDNA fragments in 200 µL of hybridization mix was loaded onto a human Clariom S array and hybridized to probes on the array in a GeneChip Hybridization Oven 645 (Affymetrix) for 16 h at 45°C with 60 rpm rotation.
|
| Scan protocol |
GeneChips were scanned using GeneArray G7 scanner (Affymetrix) and quantified into .CEL files with image and signal intensities.
|
| Data processing |
Transcriptome Analysis Console software v4.0 (Affymetrix) was used for probe set summarization and robust multiarray average (RMA) normalization procedures.
|
| |
|
| Submission date |
Sep 27, 2018 |
| Last update date |
Feb 28, 2019 |
| Contact name |
Lingegowda Selanere Mangala |
| E-mail(s) |
lsmangala@mdanderson.org
|
| Phone |
7134470026
|
| Organization name |
M.D. Anderson Cancer Center
|
| Department |
Gynecological Oncology
|
| Street address |
1881 East Rd
|
| City |
Houston |
| State/province |
TX |
| ZIP/Postal code |
77054 |
| Country |
USA |
| |
|
| Platform ID |
GPL23159 |
| Series (1) |
| GSE120585 |
Identifying and targeting angiogenesis-related microRNAs in ovarian cancer |
|
