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Sample GSM341500 Query DataSets for GSM341500
Status Public on Mar 30, 2009
Title MRC-5-1 vs mix2
Sample type RNA
 
Channel 1
Source name MRC-5-1
Organism Homo sapiens
Characteristics Derived from normal lung tissue of a 14-week-old male fetus by J. P. Jacobs in September 1966 (Nature 227: 168-170, 1970)
Treatment protocol untreated
Growth protocol MRC-5 were cultured in DMEM (GIBICO) supplemented with 10% heat-inactivated fetal bovine serum (FBS, HyClone Laboratories, Logan, UT), 0.1 mM non-essential amino acids.
Extracted molecule total RNA
Extraction protocol Total RNA are isolated from cell pellets using TriZol reagent(Invitrogen) followed by Rneasy mini kit (Qiagen) clean-up.
Label Cy3
Label protocol Please see manufacturer's web site at http://www.agilent.com/
 
Channel 2
Source name mix2
Organism Homo sapiens
Characteristics mix2 are the mixture of CCD-1079sk and IMR90.
Treatment protocol untreated
Growth protocol CCD-1079sk and IMR90 were cultured in DMEM (GIBICO) supplemented with 10% heat-inactivated fetal bovine serum (FBS, HyClone Laboratories, Logan, UT), 0.1 mM non-essential amino acids.
Extracted molecule total RNA
Extraction protocol Total RNA are isolated from cell pellets using TriZol reagent(Invitrogen) followed by Rneasy mini kit (Qiagen) clean-up.
Label Cy5
Label protocol Please see manufacturer's web site at http://www.agilent.com/
 
 
Hybridization protocol Please see manufacturer's web site at http://www.agilent.com/
Scan protocol Please see manufacturer's web site at http://www.agilent.com/
Description Please see manufacturer's web site at http://www.agilent.com/
Data processing The microarray was scanned by Agilent G2565BA scanner,raw data were extracted by Feature Extraction Software. Signal intensity were normalized using Lowess method ,GeneSpring 10.0 . VALUE:Cy3/Cy5 signal intensity, ratio:Log2 transformed
 
Submission date Nov 12, 2008
Last update date Mar 30, 2009
Contact name Jing Liao
E-mail(s) jliao@sibs.ac.cn
Phone 86-21-54921387
Fax 86-21-54921388
Organization name Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Science
Department Institute of Biochemistry and Cell Biology
Lab LeiXiao's Lab
Street address 320 Yue Yang Road
City Shanghai
ZIP/Postal code 200031
Country China
 
Platform ID GPL6480
Series (2)
GSE13462 Comparison of human ES cells, mouse ES cells, rat iPS cells and their somatic cells.
GSE13571 human MRC-5 cells' gene expression difference compared with that of the adult cells including CCD-1079sk and IMR90

Data table header descriptions
ID_REF
VALUE Cy3/Cy5 signal logratio
FLAG detection call

Data table
ID_REF VALUE FLAG
A_23_P100001 -3.5979548 P
A_23_P100011 0.45537424 P
A_23_P100022 -0.337832 A
A_23_P100056 1.1952459 P
A_23_P100074 -0.24833867 P
A_23_P100092 0.25157645 P
A_23_P100103 0.056221105 P
A_23_P100111 0.5435033 P
A_23_P100127 -1.8722286 P
A_23_P100133 -0.2974706 P
A_23_P100141 0.16014378 P
A_23_P100156 -0.15358451 P
A_23_P100177 -0.6812272 A
A_23_P100189 -0.094620705 P
A_23_P100196 0.14334397 P
A_23_P100203 0.12713255 P
A_23_P100220 -0.3267127 P
A_23_P100240 -0.8020534 P
A_23_P10025 0.08865255 P
A_23_P100263 -0.7805233 P

Total number of rows: 41000

Table truncated, full table size 1037 Kbytes.




Supplementary file Size Download File type/resource
GSM341500.txt.gz 15.3 Mb (ftp)(http) TXT
Processed data included within Sample table

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