|
| Status |
Public on Oct 10, 2019 |
| Title |
h-PBMC: Zol. (+) |
| Sample type |
RNA |
| |
|
| Source name |
h-PBMC
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: human peripheral blood mononuclear cells
|
| Treatment protocol |
h-PBMC were incuvated for 8 hours with 5 mM of Zoledronate (Zol. (+)) or without (Zol. (-)).
|
| Growth protocol |
h-PBMC were cultured in the presence of 10% FBS.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Extraction of total RNA was performed by Rneasy mini kit (Qiagen).
|
| Label |
biotin
|
| Label protocol |
Biotinylated cDNA was prepared from 100 ng of total RNA using GeneChip WT PLUS Reagent Kit（Thermo Fisher Scientific).
|
| |
|
| Hybridization protocol |
Following fragmentation, 2 μg of Single-Stranded cDNA was hybridized for 16 hrs at 45˚C on Clariom S Array, Human. Arrays was washed and stained in the GeneChip Fluidics Station 450 (Thermo Fisher Scientific).
|
| Scan protocol |
Clariom S array was scanned using GeneChip Scanner 3000 7G.
|
| Data processing |
The data were analyzed with Expression Console Software 1.4 offered SST-RMA for gene level analysis using Thermo Fisher Scientific default analysis settings.
|
| |
|
| Submission date |
Oct 10, 2018 |
| Last update date |
Oct 11, 2019 |
| Contact name |
Reiko Takimoto |
| E-mail(s) |
reiko.t@dent.showa-u.ac.jp
|
| Phone |
0337848163
|
| Organization name |
Showa University, School of Dentistry
|
| Department |
Department of Biochemistry
|
| Street address |
1-5-8
|
| City |
Shinagawa |
| State/province |
Tokyo |
| ZIP/Postal code |
148555 |
| Country |
Japan |
| |
|
| Platform ID |
GPL23159 |
| Series (1) |
| GSE121063 |
The effects of Zoledronate on human peripheral blood mononuclear cells |
|
