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Sample GSM343159 Query DataSets for GSM343159
Status Public on Jun 02, 2009
Title working myocardium _ stage e17.5 _ biorep2
Sample type RNA
 
Source name working myocardium, stage e17.5, biorep2
Organism Mus musculus
Characteristics working myocardium
stage: E17.5
Growth protocol normal development
Extracted molecule total RNA
Extraction protocol For every sample, transgenic hearts were dissected, the GFP-positive tissues pooled, dissociated to obtain single cells, and purified using FACS. 5000 cells per sample were gated and used for RNA isolation (Macherey-Nagel, nucleospin II). RNA was subjected to 2 rounds of linear amplification and biotin labeling (Ambion). Within each group 6 labeled antisense RNA samples were hybridized to separate Illumina Mouse-Ref-6 v1.1 BeadChips.
Label biotin
Label protocol RNA was subjected to 2 rounds of linear amplification and biotin labeling (Ambion). Within each group 6 labeled antisense RNA samples were hybridized to separate Illumina Mouse-Ref-6 v1.1 BeadChips.
 
Hybridization protocol standard Illumina hybridization protocol
Scan protocol standard Illumina hybridization protocol
Description W2_E17.5
Data processing Data were processed using the R/Limma package (Bioconductor). Data were normalized using VSN transformation.
 
Submission date Nov 14, 2008
Last update date May 28, 2009
Contact name Henk Buermans
E-mail(s) h.buermans@lumc.nl
Phone +31715268558
Organization name Leiden University Medical Center
Department Human Genetics
Lab Leiden Genome Technology Center
Street address Albinusdreef 2
City Leiden
State/province ZH
ZIP/Postal code 2300 RC
Country Netherlands
 
Platform ID GPL6105
Series (1)
GSE13614 Expression profiling of the forming atrioventricular node using a novel Tbx3-based node-specific transgenic reporter

Data table header descriptions
ID_REF
VALUE Ln intensity values, normalized

Data table
ID_REF VALUE
ILMN_2431248 5.9716
ILMN_3160339 3.8304
ILMN_3161159 5.3969
ILMN_2758597 4.0440
ILMN_2644469 14.5385
ILMN_2656393 7.6265
ILMN_3160634 3.8258
ILMN_3161394 14.4878
ILMN_2610322 4.0517
ILMN_2741606 4.8149
ILMN_1247267 9.2282
ILMN_2594811 5.7360
ILMN_2689425 0.8903
ILMN_2745565 2.8132
ILMN_1212992 4.6813
ILMN_2629183 3.7470
ILMN_1251092 8.0180
ILMN_2715803 10.4212
ILMN_2762509 7.3677
ILMN_2496817 8.4532

Total number of rows: 46632

Table truncated, full table size 914 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

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