|
| Status |
Public on Oct 27, 2009 |
| Title |
VAP 1 KO 2 |
| Sample type |
RNA |
| |
|
| Source name |
mouse B16 melanoma tissue
|
| Organism |
Mus musculus |
| Characteristics |
female C57/BL/6 VAP-1 -/- mouse
tumor nodule
|
| Growth protocol |
B16-F10-luc-G5 melanoma cells (Xenogen) were subcutaneously injected into abdominal area of anesthetized mice. After 10 days, mice were euthanized and tumors were dissected.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
total RNA was isolated from dissected tumors using Qiagen RNA extraction kit
|
| Label |
Biotin
|
| Label protocol |
100 ng total RNA was amplified with Ambion's Illumina RNA TotalPrep Amplification kit. IVT reaction was carried out overnight (14h) and cRNA was biotinylated.
|
| |
|
| Hybridization protocol |
1500 ng of each sample was hybridized to chips at 58 °C overnight (18 h) according to Illumina Whole-Genome Gene Expression with IntelliHyb -protocol, revision B
|
| Scan protocol |
Chips were scanned with Illumina BeadArray Reader with default setting of the scanner.
|
| Description |
none
|
| Data processing |
Data were analyzed using R2 bioconductor with quantile normalisation method.
|
| |
|
| Submission date |
Nov 17, 2008 |
| Last update date |
Oct 27, 2009 |
| Contact name |
Kaisa Auvinen |
| E-mail(s) |
kakoski@utu.fi
|
| Organization name |
University of Turku
|
| Department |
Medicity Research Laboratory
|
| Street address |
Tykistökatu 6
|
| City |
Turku |
| ZIP/Postal code |
20520 |
| Country |
Finland |
| |
|
| Platform ID |
GPL6105 |
| Series (1) |
| GSE13626 |
Comparison of gene expression in melanoma of wild-type and VAP-1 -/- mice |
|
