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Sample GSM343449 Query DataSets for GSM343449
Status Public on Dec 04, 2008
Title Glia SOD1G93Atg rep3
Sample type RNA
 
Source name Glia from pup transgenic for SOD1G93A_replicate3
Organism Mus musculus
Characteristics p1-p3
Extracted molecule total RNA
Extraction protocol P1-P3 mouse pups transgenic for SOD1G93A, SOD1WT or non-transgenic pups were sacrificed by using an approved method of euthanasia. Under a dissection microscope, the parenquima were isolated and the meninges were carefully stripped away with fine forceps. The tissue was then dissected into small pieces and transferred to a solution containing 12ml of HBSS, 1.5 ml of trypsin (GIBCO) and 1% DNAse (Sigma) and incubated at 37ºC for 15 min, swirling the mixture periodically. The supernatant containing the dissociated cells was collected and 3ml of serum was added to inhibit the trypsin. Cells were then centrifuged at 1000 rpm for 5 min, resuspended in Glia medium: (Minimun Essential Medium with Earl’s salts (GIBCO), D-(+)-Glucose 20% (Sigma), Penicillin-streptomycin (GIBCO), 10% Horse Serum (GIBCO)) and plated at the concentration of 80,000 cells per mL in T75 flasks (Falcon).
Label biotin
Label protocol Standard procedure outlined by the Illumina TotalPrep RNA Amplification Kit.
 
Hybridization protocol Standard procedure outlined by the Illumina TotalPrep RNA Amplification Kit.
Scan protocol Arrays were read by Illumina Bead Array Reader. Analysis was done using the Illumina Bead Studio Program.
Description B6SJL-Tg(SOD1*G93A)2Gur/J
Data processing Average' Normalization setting was used in BeadStudio
 
Submission date Nov 18, 2008
Last update date Nov 18, 2008
Contact name Gabriella Lutz Boulting
E-mail(s) boulting@fas.harvard.edu
Organization name Harvard University
Department Stem Cell and Regenerative Biology
Lab Eggan
Street address 7 Divinity Ave. rm 423
City Cambridge
State/province MA
ZIP/Postal code 02138
Country USA
 
Platform ID GPL6103
Series (1)
GSE13643 Identification of candidate genes involved in SOD1G93A glial toxicity

Data table header descriptions
ID_REF
VALUE average normalization, default BeadStudio setting
Avg_NBEADS
BEAD_STDERR
Detection Pval

Data table
ID_REF VALUE Avg_NBEADS BEAD_STDERR Detection Pval
ILMN_2735294 59.74226 32 4.941585 0
ILMN_1238136 -0.2314074 37 1.682332 0.4918444
ILMN_1248788 22.22926 37 3.102499 0
ILMN_2707227 6.664047 36 1.963203 0.04391468
ILMN_2721178 161.1959 41 6.937553 0
ILMN_1227723 123.9406 32 8.449384 0
ILMN_2729028 863.5289 30 47.226 0
ILMN_2602282 273.1618 26 15.81124 0
ILMN_2624326 235.0876 34 10.99755 0
ILMN_2730714 2437.224 32 106.2558 0
ILMN_3162224 631.4473 51 27.37401 0
ILMN_2773981 443.6387 36 17.05063 0
ILMN_2771349 621.16 39 40.89964 0
ILMN_1215157 428.3614 36 24.48698 0
ILMN_1216623 302.4466 47 15.2731 0
ILMN_2634566 289.3148 48 11.22548 0
ILMN_2763028 140.2918 34 9.323498 0
ILMN_1228777 6.887897 32 1.848413 0.04265998
ILMN_2737647 -1.06166 27 1.447306 0.5771644
ILMN_2744574 0.3809779 41 1.660751 0.412798

Total number of rows: 24613

Table truncated, full table size 961 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

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