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Sample GSM343451 Query DataSets for GSM343451
Status Public on Dec 04, 2008
Title Glia WTSOD1tg rep2
Sample type RNA
 
Source name Glia from pup transgenic for WT SOD1_replicate2
Organism Mus musculus
Characteristics p1-p3
Extracted molecule total RNA
Extraction protocol P1-P3 mouse pups transgenic for SOD1G93A, SOD1WT or non-transgenic pups were sacrificed by using an approved method of euthanasia. Under a dissection microscope, the parenquima were isolated and the meninges were carefully stripped away with fine forceps. The tissue was then dissected into small pieces and transferred to a solution containing 12ml of HBSS, 1.5 ml of trypsin (GIBCO) and 1% DNAse (Sigma) and incubated at 37ºC for 15 min, swirling the mixture periodically. The supernatant containing the dissociated cells was collected and 3ml of serum was added to inhibit the trypsin. Cells were then centrifuged at 1000 rpm for 5 min, resuspended in Glia medium: (Minimun Essential Medium with Earl’s salts (GIBCO), D-(+)-Glucose 20% (Sigma), Penicillin-streptomycin (GIBCO), 10% Horse Serum (GIBCO)) and plated at the concentration of 80,000 cells per mL in T75 flasks (Falcon).
Label biotin
Label protocol Standard procedure outlined by the Illumina TotalPrep RNA Amplification Kit.
 
Hybridization protocol Standard procedure outlined by the Illumina TotalPrep RNA Amplification Kit.
Scan protocol Arrays were read by Illumina Bead Array Reader. Analysis was done using the Illumina Bead Studio Program.
Description B6SJL-Tg(SOD1)2Gur/J
Data processing Average' Normalization setting was used in BeadStudio
 
Submission date Nov 18, 2008
Last update date Nov 18, 2008
Contact name Gabriella Lutz Boulting
E-mail(s) boulting@fas.harvard.edu
Organization name Harvard University
Department Stem Cell and Regenerative Biology
Lab Eggan
Street address 7 Divinity Ave. rm 423
City Cambridge
State/province MA
ZIP/Postal code 02138
Country USA
 
Platform ID GPL6103
Series (1)
GSE13643 Identification of candidate genes involved in SOD1G93A glial toxicity

Data table header descriptions
ID_REF
VALUE average normalization, default BeadStudio setting
Avg_NBEADS
BEAD_STDERR
Detection Pval

Data table
ID_REF VALUE Avg_NBEADS BEAD_STDERR Detection Pval
ILMN_2735294 64.9634 39 5.67244 0
ILMN_1238136 -0.5425698 36 1.43704 0.5327456
ILMN_1248788 23.80305 33 2.971954 0
ILMN_2707227 7.549251 36 1.718886 0.01889169
ILMN_2721178 163.4758 28 7.365735 0
ILMN_1227723 95.49596 33 7.823274 0
ILMN_2729028 1022.477 30 49.53924 0
ILMN_2602282 325.671 48 16.32305 0
ILMN_2624326 304.1411 40 14.16241 0
ILMN_2730714 2487.713 24 145.8407 0
ILMN_3162224 682.6384 49 27.04444 0
ILMN_2773981 432.1093 44 19.43939 0
ILMN_2771349 544.2418 50 37.49836 0
ILMN_1215157 359.7085 30 15.87721 0
ILMN_1216623 271.9619 40 10.35524 0
ILMN_2634566 295.3803 26 24.72231 0
ILMN_2763028 169.7637 23 11.43916 0
ILMN_1228777 7.405302 37 2.241959 0.01889169
ILMN_2737647 1.868623 36 1.635948 0.256927
ILMN_2744574 -2.072136 28 1.787417 0.7191436

Total number of rows: 24613

Table truncated, full table size 959 Kbytes.




Supplementary data files not provided
Processed data included within Sample table

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