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Status |
Public on Jun 30, 2009 |
Title |
Crhr1-ko, 1h, + stress, technical replicate 1 |
Sample type |
RNA |
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Source name |
pituitaries of Crhr1-ko mice, 30 min restraint stress, sacrificed 1h after stress begin
|
Organism |
Mus musculus |
Characteristics |
2-3 months of age, pooled together two pituitaries per group
|
Treatment protocol |
All animals were injected i.p. with metyrapone, 12h (150 mg/kg b.w.) and directly (100 mg/kg b.w.) before 30 min of restraint stress. Basal blood samples were taken by orbital sinus puncture between the second injection and the stress.
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA of prepared pituitaries was extracted using the TRIzol reagent (Invitrogen) according to manufacturer´s instruction. RNA quality was analyzed by gel electrophoresis.
|
Label |
Cy3
|
Label protocol |
One round of linear amplification with standard protocol Amino Allyl MessageAmp RNA Amplification Kit (Ambion); Dye coupling with Cy3
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Hybridization protocol |
Standard Protocol Illumina Sentrix 6 BeadChip microarrays
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Scan protocol |
StandardWithBackground; ColorChannel = Green; automatic Illumina outlier removal;
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Description |
Mice were injected with metyrapone 12h (150 mg/kg b.w.) and directly (100 mg/kg b.w.) before 30 min of restraint stress. 1h after stress begin animals were sacrificed and pituitaries prepared and frozen.
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Data processing |
The raw data were processed using cubic spline normalization of the Illumina BeadStudio Gene Expression Module Version 2.3.47
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Submission date |
Nov 19, 2008 |
Last update date |
Dec 08, 2008 |
Contact name |
Cornelia Graf |
Organization name |
MPI of Psychiatry
|
Street address |
Kraepelinstr. 2-10
|
City |
Munich |
ZIP/Postal code |
80804 |
Country |
Germany |
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|
Platform ID |
GPL4234 |
Series (1) |
GSE13665 |
Pituitaries of basal and stressed Crhr1 wild type and knockout mice |
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