|
| Status |
Public on Jun 30, 2009 |
| Title |
Crhr1-ko, 3h, + stress, technical replicate 1 |
| Sample type |
RNA |
| |
|
| Source name |
pituitaries of Crhr1-ko mice, 30 min restraint stress, sacrificed 1h after stress begin
|
| Organism |
Mus musculus |
| Characteristics |
2-3 months of age, pooled together two pituitaries per group
|
| Treatment protocol |
All animals were injected i.p. with metyrapone, 12h (150 mg/kg b.w.) and directly (100 mg/kg b.w.) before 30 min of restraint stress. Basal blood samples were taken by orbital sinus puncture between the second injection and the stress.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA of prepared pituitaries was extracted using the TRIzol reagent (Invitrogen) according to manufacturer´s instruction. RNA quality was analyzed by gel electrophoresis.
|
| Label |
Cy3
|
| Label protocol |
One round of linear amplification with standard protocol Amino Allyl MessageAmp RNA Amplification Kit (Ambion); Dye coupling with Cy3
|
| |
|
| Hybridization protocol |
Standard Protocol Illumina Sentrix 6 BeadChip microarrays
|
| Scan protocol |
StandardWithBackground; ColorChannel = Green; automatic Illumina outlier removal;
|
| Description |
Mice were injected with metyrapone 12h (150 mg/kg b.w.) and directly (100 mg/kg b.w.) before 30 min of restraint stress. 3h after stress begin animals were sacrificed and pituitaries prepared and frozen.
|
| Data processing |
The raw data were processed using cubic spline normalization of the Illumina BeadStudio Gene Expression Module Version 2.3.47
|
| |
|
| Submission date |
Nov 19, 2008 |
| Last update date |
Dec 08, 2008 |
| Contact name |
Cornelia Graf |
| Organization name |
MPI of Psychiatry
|
| Street address |
Kraepelinstr. 2-10
|
| City |
Munich |
| ZIP/Postal code |
80804 |
| Country |
Germany |
| |
|
| Platform ID |
GPL4234 |
| Series (1) |
| GSE13665 |
Pituitaries of basal and stressed Crhr1 wild type and knockout mice |
|
