|
Status |
Public on Jun 23, 2021 |
Title |
cord blood GDM_18 |
Sample type |
genomic |
|
|
Source name |
cord blood
|
Organism |
Homo sapiens |
Characteristics |
disease state: GDM neonate gender: female gestational age (wks): 39 birth weight (g): 3230 neonatal 1 hour plasma glucose level (mg/dl): NA tissue: GDM cord blood
|
Extracted molecule |
genomic DNA |
Extraction protocol |
genomic DNA was extracted and purified from cord blood using QIAsymphony DNA Midi Kit (96) according to standard instructions
|
Label |
Cy5 and Cy3
|
Label protocol |
Standard Illumina Protocol
|
|
|
Hybridization protocol |
bisulphite converted DNA was amplified, fragmented and hybridised to Illumina Infinium Human Methylation EPIC Beadchip using standard Illumina protocol
|
Scan protocol |
Arrays were imaged using BeadArray Reader using standard recommended Illumina scanner (iScan) setting
|
Data processing |
GenomeStudio software v1.9.0 The normalization method of uploaded dataset is dependent on GenomeStudio Methylation Module normalization algorithms. We performed normalization to internal controls and background subtraction.
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|
|
Submission date |
Nov 01, 2018 |
Last update date |
Jun 23, 2021 |
Contact name |
Tomoko Kawai |
E-mail(s) |
kawai-tm@ncchd.go.jp
|
Organization name |
National Research Institute for Child Health and Development
|
Department |
Department of Maternal-Fetal Biology
|
Street address |
2-10-1 Okura
|
City |
Setagaya |
State/province |
Tokyo |
ZIP/Postal code |
1578535 |
Country |
Japan |
|
|
Platform ID |
GPL21145 |
Series (1) |
GSE122086 |
Genome wide methylation of cord blood from gestational diabetes mellitus |
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