|
| Status |
Public on Aug 01, 2009 |
| Title |
Lymph nodes_Uninfected_R:04 |
| Sample type |
RNA |
| |
|
| Source name |
uninfected lymph nodes
|
| Organism |
Bos taurus |
| Characteristics |
Infection: none
|
| Treatment protocol |
Not applicable.
|
| Growth protocol |
Not applicable.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Lymph node tissue was frozen in liquid nitrogen and homogenized using a mortar and pistil. Total RNA was isolated using the RNeasy Kit by Qiagen (order number 74106, RNeasy, Qiagen, Hombrechtikon, Switzerland). Immediately upon homogenization the samples were taken up in RLT buffer with 1% β-mercaptoethanol (Sigma, Buchs, Switzerland). DNA was digested using RNase-free DNase (Qiagen, order number 79254) at room temperature for 15 minutes.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
|
| |
|
| Hybridization protocol |
Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip Bovine Genome Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
|
| Scan protocol |
Affymetrix GeneChip Scanner 3000.
|
| Description |
Biological replicate 4 of 6
|
| Data processing |
The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 500.
|
| |
|
| Submission date |
Dec 05, 2008 |
| Last update date |
Feb 20, 2009 |
| Contact name |
Hubert Rehrauer |
| E-mail(s) |
Hubert.Rehrauer@fgcz.ethz.ch
|
| Organization name |
ETH Zurich / University of Zurich
|
| Department |
FGCZ
|
| Street address |
Winterthurerstr. 190
|
| City |
Zurich |
| ZIP/Postal code |
8057 |
| Country |
Switzerland |
| |
|
| Platform ID |
GPL2112 |
| Series (2) |
| GSE13852 |
Expression profiling of Bos taurus lymph nodes upon infection with Ovine Herpesvirus 2 |
| GSE13854 |
Expression profiling of the host and the Ovine Herpesvirus 2 pathogen during malignant catarrhal fever of cattle |
|
