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Sample GSM349929 Query DataSets for GSM349929
Status Public on Aug 10, 2009
Title alveolar macrophages, smoker 114
Sample type RNA
 
Source name alveolar macrophages obtained from bronchoalveolar lavage
Organism Homo sapiens
Characteristics Age: 50
Sex: F
Ancestry: African
Smoking status: smoker, 22 pack-years
Extracted molecule total RNA
Extraction protocol Trizol extraction and RNAeasy clean-up of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 microg total RNA (Expression Analysis Technical Manual, 701022 Rev.2, Affymetrix).
 
Hybridization protocol Following fragmentation, 15 microg of cRNA were hybridized for 16 hr at 45C on GeneChip HG-U133 Plus 2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the GeneChip Scanner 3000 7G.
Description S-114_AM
Data processing The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
 
Submission date Dec 10, 2008
Last update date May 22, 2013
Contact name Yael Strulovici-Barel
E-mail(s) yas2003@med.cornell.edu
Organization name Weill Cornell Medical College
Department Department of Genetic Medicine
Lab Crystal
Street address 1300 York Avenue
City New York
State/province NY
ZIP/Postal code 10021
Country USA
 
Platform ID GPL570
Series (3)
GSE13896 Smoking-dependent Reprogramming of Alveolar Macrophage Polarization: Implication for Pathogenesis of COPD
GSE13931 Disparate Oxidant-related Gene Expression of Human Small Airway Epithelium Compared to Autologous Alveolar Macrophages
GSE20250 CNV Polymorphisms Modulate the Expression of GST Subtypes in the Human Small Airway Epithelium and Alveolar Macrophages
Relations
Reanalyzed by GSE46903

Data table header descriptions
ID_REF
VALUE Signal
ABS_CALL indicating whether the transcript was present (P), absent (A), or marginal (M)
DETECTION P-VALUE

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 265.155 P 0.00124642
AFFX-BioB-M_at 393.169 P 4.42873e-05
AFFX-BioB-3_at 206.379 P 0.000195116
AFFX-BioC-5_at 289.76 P 0.000258358
AFFX-BioC-3_at 300.217 P 0.000126798
AFFX-BioDn-5_at 1779.38 P 4.42873e-05
AFFX-BioDn-3_at 4625.03 P 7.00668e-05
AFFX-CreX-5_at 12930.3 P 4.42873e-05
AFFX-CreX-3_at 19311.6 P 4.42873e-05
AFFX-DapX-5_at 26.1499 A 0.617401
AFFX-DapX-M_at 16.5205 A 0.58862
AFFX-DapX-3_at 6.5795 A 0.937071
AFFX-LysX-5_at 2.63792 A 0.868639
AFFX-LysX-M_at 27.9431 A 0.617401
AFFX-LysX-3_at 18.6488 A 0.313708
AFFX-PheX-5_at 4.71124 A 0.921998
AFFX-PheX-M_at 17.6583 A 0.544587
AFFX-PheX-3_at 10.2587 A 0.876428
AFFX-ThrX-5_at 6.46231 A 0.957038
AFFX-ThrX-M_at 5.55568 A 0.794268

Total number of rows: 54675

Table truncated, full table size 1628 Kbytes.




Supplementary file Size Download File type/resource
GSM349929.CEL.gz 8.1 Mb (ftp)(http) CEL
GSM349929.CHP.gz 13.3 Mb (ftp)(http) CHP
Processed data included within Sample table
Processed data provided as supplementary file

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