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Sample GSM349932 Query DataSets for GSM349932
Status Public on Aug 10, 2009
Title alveolar macrophages, smoker 122
Sample type RNA
 
Source name alveolar macrophages obtained from bronchoalveolar lavage
Organism Homo sapiens
Characteristics Age: 36
Sex: M
Ancestry: Hispanic
Smoking status: smoker, 3 pack-years
Extracted molecule total RNA
Extraction protocol Trizol extraction and RNAeasy clean-up of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 2 microg total RNA (Expression Analysis Technical Manual, 701022 Rev.2, Affymetrix).
 
Hybridization protocol Following fragmentation, 15 microg of cRNA were hybridized for 16 hr at 45C on GeneChip HG-U133 Plus 2.0. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using the GeneChip Scanner 3000 7G.
Description S-122_AM
Data processing The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method.
 
Submission date Dec 10, 2008
Last update date May 22, 2013
Contact name Yael Strulovici-Barel
E-mail(s) yas2003@med.cornell.edu
Organization name Weill Cornell Medical College
Department Department of Genetic Medicine
Lab Crystal
Street address 1300 York Avenue
City New York
State/province NY
ZIP/Postal code 10021
Country USA
 
Platform ID GPL570
Series (3)
GSE13896 Smoking-dependent Reprogramming of Alveolar Macrophage Polarization: Implication for Pathogenesis of COPD
GSE13931 Disparate Oxidant-related Gene Expression of Human Small Airway Epithelium Compared to Autologous Alveolar Macrophages
GSE20250 CNV Polymorphisms Modulate the Expression of GST Subtypes in the Human Small Airway Epithelium and Alveolar Macrophages
Relations
Reanalyzed by GSE46903

Data table header descriptions
ID_REF
VALUE Signal
ABS_CALL indicating whether the transcript was present (P), absent (A), or marginal (M)
DETECTION P-VALUE

Data table
ID_REF VALUE ABS_CALL DETECTION P-VALUE
AFFX-BioB-5_at 206.55 P 0.000581214
AFFX-BioB-M_at 344.545 P 5.16732e-05
AFFX-BioB-3_at 219.532 P 0.000581214
AFFX-BioC-5_at 283.091 P 7.00668e-05
AFFX-BioC-3_at 333.812 P 0.000296708
AFFX-BioDn-5_at 1946.08 P 4.42873e-05
AFFX-BioDn-3_at 4098.41 P 0.000126798
AFFX-CreX-5_at 10804.4 P 5.16732e-05
AFFX-CreX-3_at 15819.9 P 4.42873e-05
AFFX-DapX-5_at 3.5678 A 0.772364
AFFX-DapX-M_at 34.5754 A 0.368438
AFFX-DapX-3_at 21.5676 A 0.737173
AFFX-LysX-5_at 2.74329 A 0.916426
AFFX-LysX-M_at 42.3256 A 0.327079
AFFX-LysX-3_at 20.0908 A 0.470241
AFFX-PheX-5_at 33.4751 A 0.175328
AFFX-PheX-M_at 2.78115 A 0.973889
AFFX-PheX-3_at 20.8342 A 0.52976
AFFX-ThrX-5_at 2.08936 A 0.921998
AFFX-ThrX-M_at 1.84871 A 0.887535

Total number of rows: 54675

Table truncated, full table size 1634 Kbytes.




Supplementary file Size Download File type/resource
GSM349932.CEL.gz 8.1 Mb (ftp)(http) CEL
GSM349932.CHP.gz 13.3 Mb (ftp)(http) CHP
Processed data included within Sample table
Processed data provided as supplementary file

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