|
| Status |
Public on Dec 05, 2018 |
| Title |
PBMC-Macrophage #2 |
| Sample type |
RNA |
| |
|
| Source name |
PBMC-Macrophage #2
|
| Organism |
Homo sapiens |
| Characteristics |
subject #: 299
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using the RNeasy Mini kit (QIAGEN, Hilden, Germany) as per manufacturer's instructions
|
| Label |
biotin
|
| Label protocol |
standard Affymetrix protocol
|
| |
|
| Hybridization protocol |
Whole-genome gene expression profiling was performed using the ClariomTM S Pico human gene expression array. GeneChipTM Fluidics Station 450 performed all liquid handling steps
|
| Scan protocol |
Chips were scanned with the GeneChip Scanner 3000 7G (Affymetrix Inc.).
|
| Description |
Gene Expression (Clarion_S_Human Array) PBMC-Macrophage #2
|
| Data processing |
Quality control (QC) was performed using the manufacturer’s software Transcriptome Analysis Console (TAC) v4.0 (Affymetrix Inc.), as per manufacturer's instructions (PCA, labeling controls, hybridization controls, Pos vs. Neg AUC, and Signal Box Plot) with no indication of outliers. Normalization was performed according to a Robust Multi-chip Analysis (RMA) approach.
|
| |
|
| Submission date |
Dec 04, 2018 |
| Last update date |
Dec 05, 2018 |
| Contact name |
Roy H. Perlis |
| E-mail(s) |
rperlis@mgh.harvard.edu
|
| Organization name |
Massachusetts General Hospital
|
| Department |
Center for Genomic Medicine
|
| Lab |
Perlis
|
| Street address |
185 Cambridge Street
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02114 |
| Country |
USA |
| |
|
| Platform ID |
GPL23159 |
| Series (1) |
| GSE123349 |
Expression data from iPSCs, iPSC-derived neurons, PBMC-derived Monocytes, PBMC-derived Macrophages, PBMC-derived induced microglia |
|
