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Status |
Public on Mar 01, 2019 |
Title |
22Rv1_siControl_Veh |
Sample type |
RNA |
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|
Source name |
prostate cancer
|
Organism |
Homo sapiens |
Characteristics |
cell line: 22Rv1 sirna: siControl ligand: Vehicle
|
Treatment protocol |
Cells were transfected with each siRNA 48 h before hormone treatment. Cells were treated with vehicle or dihydrotestosterone (DHT) (10 nM) for 24 h.
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Growth protocol |
22Rv1 cells were cultures in RPMI 1640 containing 10% FBS. Before hormone treatment, cells were incubated in Phenol red-free RPMI 1640 containing 2.5% charcoal/dextran treated FBS for three days.
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNAs were extracted using ISOGEN (Nippon gene) according to the manufacturer's instructions.
|
Label |
biotin
|
Label protocol |
Total RNA was labeled employing the Affymetrix GeneChip Whole Transcript (WT) Terminal Labeling kit according to the manufacturer’s instructions
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Hybridization protocol |
The samples were hybridized to Human Clariom S-arrays according to standardized protocols from Affymetrix.
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Scan protocol |
The samples were scanned on a GeneChip Scanner 3000 7G (Affymetrix).
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Description |
Gene expression data from 22Rv1 cells treated with vehicle (ethanol) after transfected with siControl
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Data processing |
The microarray data were preprocessed with Affymetrix Expression Console software using Affymetrix default Robust Multichip Analysis (RMA) sketch algorithm workflow.
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Submission date |
Dec 07, 2018 |
Last update date |
Mar 01, 2019 |
Contact name |
Ken-ichi Takayama |
Organization name |
Tokyo Metropolitan Institute of Gerontology
|
Street address |
Sakaecho
|
City |
Itabashi-ku |
ZIP/Postal code |
173-0015 |
Country |
Japan |
|
|
Platform ID |
GPL23159 |
Series (1) |
GSE123517 |
Effects of OCT1 knockdown in castration-resistant prostate cancer cells |
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