|
| Status |
Public on Mar 01, 2019 |
| Title |
22Rv1_siOCT1_DHT |
| Sample type |
RNA |
| |
|
| Source name |
prostate cancer
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: 22Rv1
sirna: siOCT1
ligand: DHT 10 nM
|
| Treatment protocol |
Cells were transfected with each siRNA 48 h before hormone treatment. Cells were treated with vehicle or dihydrotestosterone (DHT) (10 nM) for 24 h.
|
| Growth protocol |
22Rv1 cells were cultures in RPMI 1640 containing 10% FBS. Before hormone treatment, cells were incubated in Phenol red-free RPMI 1640 containing 2.5% charcoal/dextran treated FBS for three days.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNAs were extracted using ISOGEN (Nippon gene) according to the manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Total RNA was labeled employing the Affymetrix GeneChip Whole Transcript (WT) Terminal Labeling kit according to the manufacturer’s instructions
|
| |
|
| Hybridization protocol |
The samples were hybridized to Human Clariom S-arrays according to standardized protocols from Affymetrix.
|
| Scan protocol |
The samples were scanned on a GeneChip Scanner 3000 7G (Affymetrix).
|
| Description |
Gene expression data from 22Rv1 cells treated with DHT 10 nM after trasfected with siOCT1
|
| Data processing |
The microarray data were preprocessed with Affymetrix Expression Console software using Affymetrix default Robust Multichip Analysis (RMA) sketch algorithm workflow.
|
| |
|
| Submission date |
Dec 07, 2018 |
| Last update date |
Mar 01, 2019 |
| Contact name |
Ken-ichi Takayama |
| Organization name |
Tokyo Metropolitan Institute of Gerontology
|
| Street address |
Sakaecho
|
| City |
Itabashi-ku |
| ZIP/Postal code |
173-0015 |
| Country |
Japan |
| |
|
| Platform ID |
GPL23159 |
| Series (1) |
| GSE123517 |
Effects of OCT1 knockdown in castration-resistant prostate cancer cells |
|
