|
Status |
Public on Apr 29, 2020 |
Title |
Ischemia 1h-3 |
Sample type |
SRA |
|
|
Source name |
Ischemia 1h_vascular
|
Organism |
Rattus norvegicus |
Characteristics |
age: 8-week-old (220-240g) treatment: Ischemia 1h tissue: superior mesenteric artery tissues (SMAs)
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was extracted from cell or animal tissue by Trizol reagent (Invitrogen) separately, The RNA quality was checked by Bioanalyzer 2200 (Aligent) and kept at -80℃.The RNA with RIN >8.0 is right for cDNA library construction. RNA libraries were prepared for sequencing using standard Illumina protocols
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
HiSeq X Ten |
|
|
Data processing |
Reads filtering under criteria removing reads with 20% of the base quality lower than 13 HISAT2.1.0 mapping to reference genome count counting Genome_build: rnor6_NCBI Supplementary_files_format_and_content: TXT file contain counts for each sample
|
|
|
Submission date |
Dec 10, 2018 |
Last update date |
Apr 29, 2020 |
Contact name |
Chenyang Duan |
E-mail(s) |
1185357051@qq.com
|
Organization name |
Third Military Medical University
|
Department |
Second Department of Research Institute of Surgery, Daping Hospital
|
Lab |
State Key Laboratory of Trauma, Burns and Combined Injury
|
Street address |
Changjiang Road, Yuzhong District
|
City |
Chongqing |
State/province |
Chongqing |
ZIP/Postal code |
400042 |
Country |
China |
|
|
Platform ID |
GPL24688 |
Series (1) |
GSE123542 |
Transcriptome Analysis of rat vascular molecular pathways linked to mitochondria in different phase of ischeia |
|
Relations |
BioSample |
SAMN10564270 |
SRA |
SRX5123237 |