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Sample GSM3506343 Query DataSets for GSM3506343
Status Public on Apr 29, 2020
Title Ischemia 1h-3
Sample type SRA
 
Source name Ischemia 1h_vascular
Organism Rattus norvegicus
Characteristics age: 8-week-old (220-240g)
treatment: Ischemia 1h
tissue: superior mesenteric artery tissues (SMAs)
Extracted molecule total RNA
Extraction protocol Total RNA was extracted from cell or animal tissue by Trizol reagent (Invitrogen) separately, The RNA quality was checked by Bioanalyzer 2200 (Aligent) and kept at -80℃.The RNA with RIN >8.0 is right for cDNA library construction.
RNA libraries were prepared for sequencing using standard Illumina protocols
 
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model HiSeq X Ten
 
Data processing Reads filtering under criteria removing reads with 20% of the base quality lower than 13
HISAT2.1.0 mapping to reference genome
count counting
Genome_build: rnor6_NCBI
Supplementary_files_format_and_content: TXT file contain counts for each sample
 
Submission date Dec 10, 2018
Last update date Apr 29, 2020
Contact name Chenyang Duan
E-mail(s) 1185357051@qq.com
Organization name Third Military Medical University
Department Second Department of Research Institute of Surgery, Daping Hospital
Lab State Key Laboratory of Trauma, Burns and Combined Injury
Street address Changjiang Road, Yuzhong District
City Chongqing
State/province Chongqing
ZIP/Postal code 400042
Country China
 
Platform ID GPL24688
Series (1)
GSE123542 Transcriptome Analysis of rat vascular molecular pathways linked to mitochondria in different phase of ischeia
Relations
BioSample SAMN10564270
SRA SRX5123237

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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