NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM350657 Query DataSets for GSM350657
Status Public on Feb 01, 2009
Title 1672_MMF
Sample type RNA
 
Source name Carotid Endarterectomy, MMF-treated
Organism Homo sapiens
Characteristics Tissue: human carotid endarterectomy from patient with a carotid artertery stenosis (>70% diameter stenosis on angiography) as defined by the North American Symptomatic Carotid Endarterectomy Trial (NACET).
Biomaterial provider Department of Vascular Surgery at the Sint Antonius Hospital, Nieuwegein, the Netherlands
Treatment protocol The patient was randomly assigned to treatment with 1000 mg mycophenolate mofetil (MMF), 2 weeks before carotid endarterectomy surgery.
Extracted molecule total RNA
Extraction protocol Total RNA molecules were isolated from N=5 consecutive 6 micron thick frozen cross-sections of the endarterectomy sample by using Trizol reagent (Invitrogen), followed by column purification with DNase-I treatment, RNeasy Micro kit (Qiagen).
Label Biotin
Label protocol The Illumina® TotalPrep™ RNA Amplification Kit (Ambion) was used for the linear amplification of mRNA molecules into antisense biotinylated cRNAs.
 
Hybridization protocol 750ng of biotinylated cRNA was hybridized onto the HumanRef-8 Expression BeadChips according to the Manufacturer.
Scan protocol According to the Manufacturer, performed by ServiceXS http://www.servicexs.com).
Description All hybridizations were performed according to the manufacturer's specifications.
Data processing Gene expression analysis was performed by using Illumina's Beadstudio v3 software, followed by a quantile inter-array normalization (R, Bioconductor).
 
Submission date Dec 12, 2008
Last update date Jan 06, 2009
Contact name Oscar Leonard Volger
E-mail(s) Stephan.schirmer@uks.eu, a.m.vanderLaan@amc.uva.nl
Organization name Academic Medical Center
Department Cardiology
Street address Meibergdreef 15
City Amsterdam
State/province Noor Holland
ZIP/Postal code 1105 AZ
Country Netherlands
 
Platform ID GPL6255
Series (1)
GSE13922 The effects of Mycophenolate treatment on the transcriptomes of patients with symptomatic carotid artery stenosis

Data table header descriptions
ID_REF
VALUE log2 normalized signal intensity

Data table
ID_REF VALUE
ILMN_10000 9.167176388
ILMN_10001 11.19963938
ILMN_10002 7.542983343
ILMN_10004 7.379364961
ILMN_10005 7.073983965
ILMN_10006 6.924782203
ILMN_10009 6.884270998
ILMN_1001 7.428014076
ILMN_10010 6.624207764
ILMN_10011 9.400424698
ILMN_10014 6.818606675
ILMN_10016 7.01883979
ILMN_10020 8.443895922
ILMN_10021 7.700697035
ILMN_10022 6.680347056
ILMN_10024 6.830669193
ILMN_10025 8.721220137
ILMN_10026 8.009845933
ILMN_10028 6.782893548
ILMN_10029 6.700777065

Total number of rows: 15988

Table truncated, full table size 352 Kbytes.




Supplementary file Size Download File type/resource
GSM350657.txt.gz 291.8 Kb (ftp)(http) TXT
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap