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Sample GSM3509271 Query DataSets for GSM3509271
Status Public on Jun 26, 2019
Title HDN 4
Sample type SRA
Source name Neutrophils isolated from peripheral blood
Organism Mus musculus
Characteristics strain: Balb/c
gender: Female
age: 8 weeks
Extracted molecule total RNA
Extraction protocol TriZol + RNeasy mini Kit (Qiagen, #74106)
KAPA Stranded RNA-Seq PolyA (KAPA Biosystems)
Library strategy RNA-Seq
Library source transcriptomic
Library selection cDNA
Instrument model Illumina HiSeq 2000
Description Neutrophil_Cnts_070109.csv
Data processing Base calling was performed with Illumina RTA Base calls were then demultiplexed and converted to FASTQ using Illumina Casava 1.8.2.
Read trimmer was done by Trimmomatic
Alignment was performed by using STAR
Read counter was analyzed using STAR counts
Differential gene expression was analyzed using DESeq2 (version 1.16.1)
Genome_build: h19 genome
Submission date Dec 11, 2018
Last update date Jun 26, 2019
Contact name Brian Hsu
Phone 5149935989
Organization name McGill University
Department Medicine
Lab Peter Siegel
Street address 1160 Pine Avenue West
City Montréal
State/province QC
ZIP/Postal code H3A1A3
Country Canada
Platform ID GPL13112
Series (1)
GSE123669 Immature low-density neutrophils exhibit metabolic flexibility that facilitates breast cancer liver metastasis
BioSample SAMN10581963
SRA SRX5126326

Supplementary data files not provided
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

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