|
| Status |
Public on Feb 09, 2009 |
| Title |
E18.5 pylorus Nkx3.2 knockout (5805KOPS) |
| Sample type |
RNA |
| |
|
| Source name |
Pylorus tissue Nkx3.2 knockout
|
| Organism |
Mus musculus |
| Characteristics |
Mouse 129SvJ X C57Bl6
Genotype Nkx3.2 CRE/CRE
|
| Treatment protocol |
Gi tract was removed and the distal segment of the stomach locaoized. This region was excised along with 1-2 mm of tissue before and behind the pyloric stricture.
|
| Growth protocol |
Heterozygous (Bapx1 CRE/+) animals were bred and embryos collected at E18.5 days. Genotyping was obtained from yolk sac DNA and segregated embryos into KO, WT,Het.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using a kit, Rneasy from Qiagen. RNA purity was assessed by Agilent 2100 Bioanalyzer.
|
| Label |
biotin
|
| Label protocol |
2 ug total RNA was used to generat biotin-labeled cRNA using a codelink iExpress kit.
|
| |
|
| Hybridization protocol |
Labeled cRNA was applied to the Codelink genome arrays (GPL2897) for 18 hrs and washed, stained using the Codelink protocol and reagents
|
| Scan protocol |
Arrays were scanned on an Axon 4000B scanner.
|
| Description |
gene expression data from distal stomach from Nkx3.2 knockout mouse
|
| Data processing |
Array images were processed using codelink software, and global median normalization used to generate normalized expression values.
|
| |
|
| Submission date |
Dec 12, 2008 |
| Last update date |
Dec 15, 2008 |
| Contact name |
Warren Eugene Zimmer |
| E-mail(s) |
wezimmer@medicine.tamhsc.edu
|
| Phone |
979-845-2896
|
| Fax |
979-862-4638
|
| Organization name |
Texas A&M Health Science Center
|
| Department |
Systems Biology and Translational Medicine
|
| Street address |
310b J. Reynolds Medical Building
|
| City |
College Station |
| State/province |
TX |
| ZIP/Postal code |
77845 |
| Country |
USA |
| |
|
| Platform ID |
GPL2897 |
| Series (1) |
| GSE13935 |
Gene expression in pylorus stomach tissue in Bapx1 (Nkx3.2) deficient mice |
|
