|
| Status |
Public on Dec 17, 2008 |
| Title |
control replicate 1 |
| Sample type |
RNA |
| |
|
| Source name |
Whole Retina
|
| Organism |
Danio rerio |
| Characteristics |
Adult zebrafish; Albino strain
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted using the Ambion RNaqueous kit per manufacturers instructions
|
| Label |
biotin
|
| Label protocol |
Affymetrix GeneChip Expression IVT Labeling Kit
|
| |
|
| Hybridization protocol |
Following fragmentation, 10ug of cRNA were hybridized on GeneChip Zebrafish Array. GeneChips were washed and stained in the Affymetrix Fluidics Station
|
| Scan protocol |
GeneChips were scanned using the Affymetrix GeneChip Scanner 3000
|
| Description |
Gene Expression for unlesioned control retina
|
| Data processing |
The data were analyzed with BRB Array Tools 3.6 using default analysis settings and global scaling for RMA normalization
|
| |
|
| Submission date |
Dec 16, 2008 |
| Last update date |
Dec 16, 2008 |
| Contact name |
Peter F. Hitchcock |
| E-mail(s) |
peterh@umich.edu
|
| Phone |
734-763-8169
|
| Fax |
734-936-7231
|
| Organization name |
University of Michigan
|
| Department |
Ophthy and Vis Sci
|
| Street address |
1000 Wall Street
|
| City |
Ann Arbor |
| State/province |
MI |
| ZIP/Postal code |
48104 |
| Country |
USA |
| |
|
| Platform ID |
GPL1319 |
| Series (1) |
| GSE13999 |
The cellular expression of midkine-a and midkine-b during retinal development and photoreceptor regeneration |
|
