|
| Status |
Public on Feb 10, 2009 |
| Title |
Sample 70: treated with frankincense oil for 3 hrs |
| Sample type |
RNA |
| |
|
| Source name |
Bladder transitional cell carcinoma J82 was obtained from ATCC (HTB-1; Manassas, VA)
|
| Organism |
Homo sapiens |
| Characteristics |
J86 cell line treated with frankincense oil for 3 hrs
|
| Biomaterial provider |
HK Lin
|
| Treatment protocol |
Cells untreated or Cells treated with 1/1000 dilution of frankincense oil (Young Living EWssential Oil, Lehi, Utah) for 1 hr prior to RNA extraction
|
| Growth protocol |
J82 cells were maintained MEM medium supplemented with 10% FBS, 0.1 mM non-essential amino acids, 1 mM sodium pyruvate, 2% MEM vitamin solution, and 100 units/ml penicillin-, and 100 µg/ml streptomycin.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated at 3 hours following exposure to frankincense oil using RNeasy® Mini total RNA isolation kit based on manufacture’s procedures.
|
| Label |
Cy3
|
| Label protocol |
250 ng of RNA from a single cell culture from each time point was labeled using the Illumina Total Prep RNA Amplification Kit following manufacturer’s directions (Ambion, Austin. TX)
|
| |
|
| Hybridization protocol |
cRNA was hybridized overnight on Illumina human Ref-8 version 3 BeadChips. Microarray chips were washed to high stringency and labeled with streptavidin -Cy3 (Amersham Biosciences, Piscataway, NJ) prior to scanning on an Illumina BeadArray Reader.
|
| Scan protocol |
Microarrays were scanned on an Illumina BeadArray Reader.
|
| Description |
None
|
| Data processing |
Initial intensity values were obtained using with Illumina Bead Studio software with background subtraction. For normalization, negative values were replaced with 1, and all values were quantile normalized using Matlab software.
|
| |
|
| Submission date |
Dec 16, 2008 |
| Last update date |
Feb 10, 2009 |
| Contact name |
Bart Frank |
| E-mail(s) |
frank@omrf.org
|
| Organization name |
OMRF
|
| Street address |
825 NE 13 St
|
| City |
Oklahoma City |
| State/province |
OK |
| ZIP/Postal code |
73103 |
| Country |
USA |
| |
|
| Platform ID |
GPL6883 |
| Series (1) |
| GSE14002 |
J82 human bladder cell line treated with frankincense oil |
|
