|
| Status |
Public on Dec 01, 2020 |
| Title |
D19_ctrl_1 |
| Sample type |
SRA |
| |
|
| Source name |
Hepatocytes
|
| Organism |
Homo sapiens |
| Characteristics |
developmental stage: day 19
tissue: liver
derived cell line: H9
cell type: Hepatocytes
treatment: ctrl
|
| Treatment protocol |
Cells were treated with 0.1% DMSO as control, or 30 nM T3/T4.
|
| Growth protocol |
Undifferentiated H9 cells were maintained on vitronectin-coated tissue culture dishes in mTeSR1 medium.A previously reported hepatocyte-like cell differentiation protocol was performed to induce H9 cells into hepatocyte cells (Mathapati, et al., 2016)
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted with the Trizol reagent.
RNA libraries were prepared for sequencing using standard Illumina protocols
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
BGISEQ-500 |
| |
|
| Data processing |
Reads were filtered and then mapped to the reference genome sequence (UCSC hg38) with HISAT
Sequences were aligned to reference genes with Bowtie2 tool
The gene expression level was determined by the RSEM software
Genome_build: UCSC hg38
Supplementary_files_format_and_content: FPKMs
|
| |
|
| Submission date |
Dec 18, 2018 |
| Last update date |
Dec 02, 2020 |
| Contact name |
liang sheng xian |
| E-mail(s) |
1024289419@qq.com
|
| Organization name |
chinese academy of science
|
| Street address |
shuangqing road
|
| City |
beijing |
| ZIP/Postal code |
100085 |
| Country |
China |
| |
|
| Platform ID |
GPL23227 |
| Series (1) |
| GSE124017 |
Transcriptional analysis of THs-responding genes during human hepatocyte development |
|
| Relations |
| BioSample |
SAMN10611038 |
| SRA |
SRX5157070 |
