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Sample GSM35258 Query DataSets for GSM35258
Status Public on Jul 01, 2005
Title UB tip 2
Sample type RNA
Source name E12.5 ureteric bud tip
Organism Mus musculus
Extracted molecule total RNA
Description Metanephric mesenchyme and ureteric buds were dissected from E12.5 mice. At this stage of development, the ureteric buds have already initiated branching morphogenesis producing four or more tips attached to one stalk. To isolate the metanephric mesenchyme, ureteric bud tip and stalk for separate analysis, embryonic kidneys were dissected and treated with DNase (100 U/ml; Boehringer Mannheim) and trypsin (1 mg/ml; Sigma) for 10 min at 37C in L-15 media. The mesenchyme was freed with minutia pins (Fine Scientific) and the tissues were transferred to L-15 with soybean trypsin inhibitor (1 mg/ml). Cross-contamination between ureteric buds and metanephric mesenchymes was ruled out by visual inspection and staining with dolichos bifloris lectin, which selectively stains the ureteric bud at this stage. Ureteric bud tips were dissected from ureteric bud stalks using minutia pins. In all, approximately 1,000 mouse ureteric bud tips, 350 mouse ureteric bud stalks, and 300 mouse mesenchymes were harvested to prepare RNA. Total RNA from ureteric buds and metanephric mesenchymes was extracted using RNeasy mini kit (Qiagen, Valencia, CA) with on-column DNase digestion according to the manufacturer´s instructions. Targets were produced using standard Affymetrix procedures from 1 ug of total RNA. 2 replicates of the target labeling process were analyzed for each sample.
Keywords = Mus musculus, metanephric mesenchyme, ureteric bud tip, ureteric bud stalk, molecular screen for spatially restricted transcripts
Submission date Nov 13, 2004
Last update date Sep 23, 2010
Contact name Kai Martin Schmidt-Ott
Organization name Max-Delbrueck Center for Molecular Medicine
Department Differentiation and Regeneration of Kidney Epithelia
Lab Kai Schmidt-Ott
Street address Robert-Roessle-Str. 10
City Berlin
ZIP/Postal code 13125
Country Germany
Platform ID GPL1261
Series (1)
GSE1983 Transcriptional Profiling of the Metanephric Kidney
Reanalyzed by GSM599002

Data table header descriptions
VALUE 'signal' a measure of the abundance of a transcript according to robust multichip analysis (RMA)

Data table
1415670_at 629.9
1415671_at 573.4
1415672_at 1951.3
1415673_at 979.1
1415674_a_at 587.9
1415675_at 440.2
1415676_a_at 2576.8
1415677_at 330.1
1415678_at 579.5
1415679_at 1578.5
1415680_at 754.3
1415681_at 812.4
1415682_at 372.9
1415683_at 1320.6
1415684_at 337.6
1415685_at 492.7
1415686_at 517.0
1415687_a_at 1099.2
1415688_at 1051.2
1415689_s_at 305.7

Total number of rows: 45101

Table truncated, full table size 737 Kbytes.

Supplementary data files not provided

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