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Sample GSM35261 Query DataSets for GSM35261
Status Public on Jul 01, 2005
Title metanephric mesenchyme 1
Sample type RNA
Source name E12.5 metanephric mesenchyme
Organism Mus musculus
Extracted molecule total RNA
Description Metanephric mesenchyme and ureteric buds were dissected from E12.5 mice. At this stage of development, the ureteric buds have already initiated branching morphogenesis producing four or more tips attached to one stalk. To isolate the metanephric mesenchyme, ureteric bud tip and stalk for separate analysis, embryonic kidneys were dissected and treated with DNase (100 U/ml; Boehringer Mannheim) and trypsin (1 mg/ml; Sigma) for 10 min at 37C in L-15 media. The mesenchyme was freed with minutia pins (Fine Scientific) and the tissues were transferred to L-15 with soybean trypsin inhibitor (1 mg/ml). Cross-contamination between ureteric buds and metanephric mesenchymes was ruled out by visual inspection and staining with dolichos bifloris lectin, which selectively stains the ureteric bud at this stage. Ureteric bud tips were dissected from ureteric bud stalks using minutia pins. In all, approximately 1,000 mouse ureteric bud tips, 350 mouse ureteric bud stalks, and 300 mouse mesenchymes were harvested to prepare RNA. Total RNA from ureteric buds and metanephric mesenchymes was extracted using RNeasy mini kit (Qiagen, Valencia, CA) with on-column DNase digestion according to the manufacturer´s instructions. Targets were produced using standard Affymetrix procedures from 1 ug of total RNA. 2 replicates of the target labeling process were analyzed for each sample.
Keywords = Mus musculus, metanephric mesenchyme, ureteric bud tip, ureteric bud stalk, molecular screen for spatially restricted transcripts
Submission date Nov 13, 2004
Last update date Sep 23, 2010
Contact name Kai Martin Schmidt-Ott
Organization name Max-Delbrueck Center for Molecular Medicine
Department Differentiation and Regeneration of Kidney Epithelia
Lab Kai Schmidt-Ott
Street address Robert-Roessle-Str. 10
City Berlin
ZIP/Postal code 13125
Country Germany
Platform ID GPL1261
Series (1)
GSE1983 Transcriptional Profiling of the Metanephric Kidney
Reanalyzed by GSM598997

Data table header descriptions
VALUE 'signal' a measure of the abundance of a transcript according to robust multichip analysis (RMA)

Data table
1415670_at 799.1
1415671_at 914.2
1415672_at 2207.7
1415673_at 179.9
1415674_a_at 694.2
1415675_at 511.1
1415676_a_at 2294.2
1415677_at 440.3
1415678_at 617.1
1415679_at 1476.9
1415680_at 688.8
1415681_at 912.4
1415682_at 340.7
1415683_at 1168.9
1415684_at 370.1
1415685_at 556.2
1415686_at 705.3
1415687_a_at 1035.9
1415688_at 1314.9
1415689_s_at 532.9

Total number of rows: 45101

Table truncated, full table size 737 Kbytes.

Supplementary data files not provided

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