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Sample GSM35282 Query DataSets for GSM35282
Status Public on Jul 01, 2005
Title UB 2
Sample type RNA
Source name E13.5 ureteric bud
Organism Rattus norvegicus
Extracted molecule total RNA
Description Metanephric mesenchyme and ureteric buds were dissected from E13.5 rats. At this stage of development, the ureteric buds have already initiated branching morphogenesis producing cleavage of the initial bulbous ureteric bud into a T-shaped tubule. To isolate the metanephric mesenchyme and ureteric bud, embryonic kidneys were dissected and treated with DNase (100 U/ml; Boehringer Mannheim) and trypsin (1 mg/ml; Sigma) for 10 min at 37C in L-15 media. The mesenchyme was freed with minutia pins (Fine Scientific) and the tissues were transferred to L-15 with soybean trypsin inhibitor (1 mg/ml). Cross-contamination between ureteric buds and metanephric mesenchymes was ruled out by visual inspection and staining with dolichos bifloris lectin, which selectively stains the ureteric bud at this stage. In all, approximately 1,200 rat ureteric buds and 240 rat mesenchymes were harvested to prepare RNA. Total RNA from ureteric buds and metanephric mesenchymes was extracted using RNeasy mini kit (Qiagen, Valencia, CA) with on-column DNase digestion according to the manufacturer´s instructions. Targets were produced using standard Affymetrix procedures from 1 ug of total RNA. 2 replicates of the target labeling process were analyzed for each sample.
Keywords = Rattus norvegicus, metanephric mesenchyme, ureteric bud, molecular screen for spatially restricted transcripts
Submission date Nov 13, 2004
Last update date Oct 28, 2005
Contact name Kai Martin Schmidt-Ott
Organization name Max-Delbrueck Center for Molecular Medicine
Department Differentiation and Regeneration of Kidney Epithelia
Lab Kai Schmidt-Ott
Street address Robert-Roessle-Str. 10
City Berlin
ZIP/Postal code 13125
Country Germany
Platform ID GPL85
Series (1)
GSE1983 Transcriptional Profiling of the Metanephric Kidney

Data table header descriptions
VALUE 'signal' a measure of the abundance of a transcript according to robust multichip analysis (RMA)

Data table
A01157cds_s_at 80.1
A03913cds_s_at 104.7
A04674cds_s_at 73.2
A07543cds_s_at 124.0
A09811cds_s_at 1707.8
A16585cds_s_at 102.9
A17753cds_s_at 100.5
A30543cds_s_at 64.4
A44407cds_at 73.9
AA108277_at 35.3
AA108308_i_at 27.6
AA108308_s_at 328.5
AA684537_at 293.7
AA684929_f_at 206.8
AA684960_f_at 255.1
AA684963_at 170.2
AA685112_at 262.4
AA685152_r_at 135.8
AA685376_f_at 144.2
AA685876_at 313.7

Total number of rows: 8799

Table truncated, full table size 162 Kbytes.

Supplementary data files not provided

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