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Sample GSM35284 Query DataSets for GSM35284
Status Public on Jul 01, 2005
Title MM 2
Sample type RNA
 
Source name E13.5 metanephric mesenchyme
Organism Rattus norvegicus
Extracted molecule total RNA
 
Description Metanephric mesenchyme and ureteric buds were dissected from E13.5 rats. At this stage of development, the ureteric buds have already initiated branching morphogenesis producing cleavage of the initial bulbous ureteric bud into a T-shaped tubule. To isolate the metanephric mesenchyme and ureteric bud, embryonic kidneys were dissected and treated with DNase (100 U/ml; Boehringer Mannheim) and trypsin (1 mg/ml; Sigma) for 10 min at 37C in L-15 media. The mesenchyme was freed with minutia pins (Fine Scientific) and the tissues were transferred to L-15 with soybean trypsin inhibitor (1 mg/ml). Cross-contamination between ureteric buds and metanephric mesenchymes was ruled out by visual inspection and staining with dolichos bifloris lectin, which selectively stains the ureteric bud at this stage. In all, approximately 1,200 rat ureteric buds and 240 rat mesenchymes were harvested to prepare RNA. Total RNA from ureteric buds and metanephric mesenchymes was extracted using RNeasy mini kit (Qiagen, Valencia, CA) with on-column DNase digestion according to the manufacturer´s instructions. Targets were produced using standard Affymetrix procedures from 1 ug of total RNA. 2 replicates of the target labeling process were analyzed for each sample.
Keywords = Rattus norvegicus, metanephric mesenchyme, ureteric bud, molecular screen for spatially restricted transcripts
 
Submission date Nov 13, 2004
Last update date Oct 28, 2005
Contact name Kai Martin Schmidt-Ott
Organization name Max-Delbrueck Center for Molecular Medicine
Department Differentiation and Regeneration of Kidney Epithelia
Lab Kai Schmidt-Ott
Street address Robert-Roessle-Str. 10
City Berlin
ZIP/Postal code 13125
Country Germany
 
Platform ID GPL85
Series (1)
GSE1983 Transcriptional Profiling of the Metanephric Kidney

Data table header descriptions
ID_REF
VALUE 'signal' a measure of the abundance of a transcript according to robust multichip analysis (RMA)

Data table
ID_REF VALUE
A01157cds_s_at 90.3
A03913cds_s_at 177.9
A04674cds_s_at 79.3
A07543cds_s_at 113.4
A09811cds_s_at 278.4
A16585cds_s_at 99.6
A17753cds_s_at 100.6
A30543cds_s_at 59.9
A44407cds_at 86.9
AA108277_at 139.8
AA108308_i_at 43.2
AA108308_s_at 404.6
AA684537_at 293.4
AA684929_f_at 217.6
AA684960_f_at 256.2
AA684963_at 296.6
AA685112_at 436.0
AA685152_r_at 170.8
AA685376_f_at 152.7
AA685876_at 359.0

Total number of rows: 8799

Table truncated, full table size 162 Kbytes.




Supplementary data files not provided

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