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Sample GSM35292 Query DataSets for GSM35292
Status Public on Jul 01, 2005
Title MM 1C
Sample type RNA
 
Source name E13.5 metanephric mesenchyme
Organism Rattus norvegicus
Extracted molecule total RNA
 
Description Metanephric mesenchyme and ureteric buds were dissected from E13.5 rats. At this stage of development, the ureteric buds have already initiated branching morphogenesis producing cleavage of the initial bulbous ureteric bud into a T-shaped tubule. To isolate the metanephric mesenchyme and ureteric bud, embryonic kidneys were dissected and treated with DNase (100 U/ml; Boehringer Mannheim) and trypsin (1 mg/ml; Sigma) for 10 min at 37C in L-15 media. The mesenchyme was freed with minutia pins (Fine Scientific) and the tissues were transferred to L-15 with soybean trypsin inhibitor (1 mg/ml). Cross-contamination between ureteric buds and metanephric mesenchymes was ruled out by visual inspection and staining with dolichos bifloris lectin, which selectively stains the ureteric bud at this stage. In all, approximately 1,200 rat ureteric buds and 240 rat mesenchymes were harvested to prepare RNA. Total RNA from ureteric buds and metanephric mesenchymes was extracted using RNeasy mini kit (Qiagen, Valencia, CA) with on-column DNase digestion according to the manufacturer´s instructions. Targets were produced using standard Affymetrix procedures from 1 ug of total RNA. 2 replicates of the target labeling process were analyzed for each sample.
Keywords = Rattus norvegicus, metanephric mesenchyme, ureteric bud, molecular screen for spatially restricted transcripts
 
Submission date Nov 13, 2004
Last update date Oct 28, 2005
Contact name Kai Martin Schmidt-Ott
Organization name Max-Delbrueck Center for Molecular Medicine
Department Differentiation and Regeneration of Kidney Epithelia
Lab Kai Schmidt-Ott
Street address Robert-Roessle-Str. 10
City Berlin
ZIP/Postal code 13125
Country Germany
 
Platform ID GPL87
Series (1)
GSE1983 Transcriptional Profiling of the Metanephric Kidney

Data table header descriptions
ID_REF
VALUE 'signal' a measure of the abundance of a transcript according to robust multichip analysis (RMA)

Data table
ID_REF VALUE
AA012646_i_at 259.6
AA012646_r_at 12.0
AA012654_at 59.5
AA012693_at 29.0
AA012699_at 33.8
AA012708_f_at 1319.3
AA012709_at 161.4
AA012719_at 95.2
AA012727_at 147.8
AA012785_at 6.6
AA012807_at 41.9
AA012808_at 46.9
AA108284_at 13.6
AA684857_s_at 1410.4
AA684879_at 237.1
AA684961_at 243.7
AA685015_at 319.9
AA685212_at 131.4
AA685258_at 146.8
AA685760_at 825.7

Total number of rows: 8789

Table truncated, full table size 176 Kbytes.




Supplementary data files not provided

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