|
| Status |
Public on Jun 01, 2020 |
| Title |
CR9_NSCs-Fetal_PC-2 |
| Sample type |
RNA |
| |
|
| Source name |
Neural Stem Cells derived from the forebrain of 15-22 weeks (EGA) fetuses that had undergone natural, spontaneous in utero death.
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Fetal forebrain
cell type: Neural Stem Cells
|
| Treatment protocol |
Not applicable
|
| Growth protocol |
Cultured with FGF and EGF in a humidified incubator at 37°C, 5% O2 and 5% CO2
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted with RNeasy® Mini kit (Qiagen) following the instructions of the manufacturer.
|
| Label |
biotin
|
| Label protocol |
cDNA was synthetized and labelled with biotin following the instructions of the manufacturer (Affymetrix, WT PLUS)
|
| |
|
| Hybridization protocol |
Hybridization was performed with independent Human Clarion-S Microarrays (Affymetrix) following Affymetrix protocol.
|
| Scan protocol |
Microarrays were scanned with Affymetrix GeneChip Scanner 7G
|
| Data processing |
Data were analyzed with Affymetrix ® GeneChip® Command Console® 2.0 software. Relevant Software parameters were the following: Anova Method: eBayes; DAGB threshold < 0.05; Pos/Neg AUC threshold: 0.7; Exploratory Grouping Analysis (all parameters): Default; Signal Space Transformation - Robust Microarray Analysis (SST-RMA) was used as the normalization method.
|
| |
|
| Submission date |
Dec 26, 2018 |
| Last update date |
Jun 02, 2020 |
| Contact name |
Luis Lopez Navas |
| Phone |
+34656523579
|
| Organization name |
Red Andaluza de diseño y traslación de Terapias Avanzadas
|
| Department |
Preclinical Research
|
| Street address |
c/ Americo Vespucio, 15, Ed. S-2
|
| City |
Sevilla |
| State/province |
Sevilla |
| ZIP/Postal code |
41092 |
| Country |
Spain |
| |
|
| Platform ID |
GPL23159 |
| Series (1) |
| GSE124361 |
Retrieval of germinal zone neural stem cells from the cerebrospinal fluid of premature infants with intraventricular hemorrhage |
|
