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Status |
Public on Jun 01, 2020 |
Title |
CR36_CD133+ |
Sample type |
RNA |
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|
Source name |
CD133+ purified Neural Stem Cells derived from cerebrospinal fluid obtained from 27-36 weeks (EGA) preterm infants. Case 3
|
Organism |
Homo sapiens |
Characteristics |
tissue: Hemorrhagic cerebrospinal fluid cell type: Neural Stem Cells
|
Treatment protocol |
Not applicable
|
Growth protocol |
CD133+ cells purified by MACS
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was extracted with RNeasy® Mini kit (Qiagen) following the instructions of the manufacturer.
|
Label |
biotin
|
Label protocol |
cDNA was synthetized and labelled with biotin following the instructions of the manufacturer (Affymetrix, WT PLUS)
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|
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Hybridization protocol |
Hybridization was performed with independent Human Clarion-S Microarrays (Affymetrix) following Affymetrix protocol.
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Scan protocol |
Microarrays were scanned with Affymetrix GeneChip Scanner 7G
|
Data processing |
Data were analyzed with Affymetrix ® GeneChip® Command Console® 2.0 software. Relevant Software parameters were the following: Anova Method: eBayes; DAGB threshold < 0.05; Pos/Neg AUC threshold: 0.7; Exploratory Grouping Analysis (all parameters): Default; Signal Space Transformation - Robust Microarray Analysis (SST-RMA) was used as the normalization method.
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Submission date |
Dec 26, 2018 |
Last update date |
Jun 02, 2020 |
Contact name |
Luis Lopez Navas |
Phone |
+34656523579
|
Organization name |
Red Andaluza de diseño y traslación de Terapias Avanzadas
|
Department |
Preclinical Research
|
Street address |
c/ Americo Vespucio, 15, Ed. S-2
|
City |
Sevilla |
State/province |
Sevilla |
ZIP/Postal code |
41092 |
Country |
Spain |
|
|
Platform ID |
GPL23159 |
Series (1) |
GSE124361 |
Retrieval of germinal zone neural stem cells from the cerebrospinal fluid of premature infants with intraventricular hemorrhage |
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