|
| Status |
Public on Apr 17, 2019 |
| Title |
Brain frontal cortex_Control_A1 |
| Sample type |
SRA |
| |
|
| Source name |
Frontal cortex
|
| Organism |
Rattus norvegicus |
| Characteristics |
tissue: Brain frontal cortex
group: Control
age: 14 weeks
|
| Treatment protocol |
Rats in acupuncture group were acupunctured at GV20 (Baihui) and GV29 (Yintang) from PND61 to PND81 every other day under anesthetized, including Control, MS.
|
| Growth protocol |
From PND1 to PND21, the maternal separation that kept the mothers from their filial generation of MS group into another cage for four hours (9:00-13:00) was conducted every day. While the mothers and litters in control group were under no disturbance until weaning. All pups were weaned at PND 21 and the males were housed by four or five per cage until adult age, and the females were eliminated.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from cell or animal tissue by Trizol reagent (Invitrogen) separately. The RNA quality was checked by Bioanalyzer 2200 (Aligent) and kept at -80℃.The RNA with RIN >8.0 is right for cDNA library construction.
RNA libraries were prepared for sequencing using standard Illumina protocols
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
HiSeq X Ten |
| |
|
| Data processing |
We applied the reads filtering towards the raw reads after sequencing to achieve the clean data following the criteria: a)10% base quality <15 b) 13% base quality <20.
Hisat2 2.0.4 mapping to reference genome
count counting
Genome_build: Rnor_6.0
Supplementary_files_format_and_content: TXT file contain counts for each sample
|
| |
|
| Submission date |
Dec 26, 2018 |
| Last update date |
Apr 17, 2019 |
| Contact name |
Yongjun Chen |
| E-mail(s) |
chyj417@126.com
|
| Organization name |
Guangzhou University of Chinese Medicine
|
| Department |
Medical College of Acu-Moxi and Rehabilitation
|
| Lab |
South China Research Center for Acupuncture and Moxibustion
|
| Street address |
232 Wai Huan East Road, University Town,Panyu District
|
| City |
Guangzhou |
| State/province |
Guangdong |
| ZIP/Postal code |
510006 |
| Country |
China |
| |
|
| Platform ID |
GPL24688 |
| Series (1) |
| GSE124387 |
Transcriptome Analysis on Maternal Separation Rats with Depression-related Manifestations Ameliorated by Electroacupuncture |
|
| Relations |
| BioSample |
SAMN10644927 |
| SRA |
SRX5180557 |
