|
| Status |
Public on Mar 12, 2019 |
| Title |
6 Fed.p110alpha.KO |
| Sample type |
SRA |
| |
|
| Source name |
Skeletal muscle
|
| Organism |
Mus musculus |
| Characteristics |
treatment: Fed
strain: C57BL/6J
Sex: male
genotype: p110alpha.KO
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from tissues using Trizol.
Library for RNA-Seq were prepared using NEBNext mRNA Sample Prep Master Mix kit (NEB), and HTG EdgeSeq mRNA sequence analysis was performed by BioPolymers Facility at Harvard Medical School.
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina HiSeq 2500 |
| |
|
| Description |
counts_table.csv
TRA00051854
|
| Data processing |
Reads were aligned to the mouse genome using STAR aligner.
Reads were counted with Subread featureCounts.
Genome_build: GRCm38
Supplementary_files_format_and_content: CSV file contains read counts.
|
| |
|
| Submission date |
Dec 26, 2018 |
| Last update date |
Mar 12, 2019 |
| Contact name |
Hui Pan |
| E-mail(s) |
Hui.Pan@joslin.harvard.edu
|
| Organization name |
Joslin Diabetes Center
|
| Department |
Bioinformatics and Biostatistics Core
|
| Street address |
1 Joslin Place
|
| City |
Boston |
| ZIP/Postal code |
02215 |
| Country |
USA |
| |
|
| Platform ID |
GPL17021 |
| Series (1) |
| GSE124394 |
Role of p110a subunit of PI3-kinase in skeletal muscle mitochondria |
|
| Relations |
| BioSample |
SAMN10646048 |
| SRA |
SRX5180706 |
