|
Status |
Public on Mar 12, 2019 |
Title |
6 Fed.p110alpha.KO |
Sample type |
SRA |
|
|
Source name |
Skeletal muscle
|
Organism |
Mus musculus |
Characteristics |
treatment: Fed strain: C57BL/6J Sex: male genotype: p110alpha.KO
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was extracted from tissues using Trizol. Library for RNA-Seq were prepared using NEBNext mRNA Sample Prep Master Mix kit (NEB), and HTG EdgeSeq mRNA sequence analysis was performed by BioPolymers Facility at Harvard Medical School.
|
|
|
Library strategy |
RNA-Seq |
Library source |
transcriptomic |
Library selection |
cDNA |
Instrument model |
Illumina HiSeq 2500 |
|
|
Description |
counts_table.csv TRA00051854
|
Data processing |
Reads were aligned to the mouse genome using STAR aligner. Reads were counted with Subread featureCounts. Genome_build: GRCm38 Supplementary_files_format_and_content: CSV file contains read counts.
|
|
|
Submission date |
Dec 26, 2018 |
Last update date |
Mar 12, 2019 |
Contact name |
Hui Pan |
E-mail(s) |
Hui.Pan@joslin.harvard.edu
|
Organization name |
Joslin Diabetes Center
|
Department |
Bioinformatics and Biostatistics Core
|
Street address |
1 Joslin Place
|
City |
Boston |
ZIP/Postal code |
02215 |
Country |
USA |
|
|
Platform ID |
GPL17021 |
Series (1) |
GSE124394 |
Role of p110a subunit of PI3-kinase in skeletal muscle mitochondria |
|
Relations |
BioSample |
SAMN10646048 |
SRA |
SRX5180706 |