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Sample GSM353253 Query DataSets for GSM353253
Status Public on Jul 01, 2009
Title Spermatozoal RNA profile from a normally fertile individual: Sample 248
Sample type RNA
 
Source name Normal Human Spermatozoa
Organism Homo sapiens
Characteristics Semen from a normally fertile donor, fertility demonstrated by fathering a child.
Extracted molecule total RNA
Extraction protocol Semen was collected then treated with Somatic Cell Lysis Buffer (0.1% sodium dodecyl sulfate [SDS], 0.5% Triton X-100) on ice for 30 minutes. Lack of somatic cell contamination and sperm count were established by microscopic examination. RNA was isolated from sample using Trizol. Samples were then treated with RNase-free DNase I
Label Biotin
Label protocol Biotinylated cRNA were prepared according to the standard Illumina protocol from total RNA (Whole-Genome Gene Expression with IntelliHyb Seal System Manual, 2006, Illumina).
 
Hybridization protocol Hybridization to Illumina Sentrix Expression BeadChip, staining and washing were carried out as described (Illumina, Wallingford).
Scan protocol Scanning procedures were carried out as described by Illumina, Wallingford.
Description Spermatozoal RNA profile from a normally fertile individual
Data processing The data were analyzed with Illumina Bead Studio. Raw data was used without further normalization due to the high inherent variability in sperm RNA contributions.
 
Submission date Dec 21, 2008
Last update date Dec 22, 2008
Contact name Stephen A Krawetz
E-mail(s) steve@compbio.med.wayne.edu
Phone 313-577-0765
URL http://compbio.med.wayne.edu
Organization name Wayne State University
Department Obstetrics and Gynecology
Street address 275 E. Hancock, CS Mott Center
City Detroit
State/province MI
ZIP/Postal code 48201
Country USA
 
Platform ID GPL6104
Series (1)
GSE14078 Normal fertile Spermatozoal RNA Profiles

Data table header descriptions
ID_REF
VALUE SIGNAL VALUE

Data table
ID_REF VALUE
ILMN_1681101 130.8264
ILMN_1703142 913.324
ILMN_1699644 165.6523
ILMN_1735038 122.099
ILMN_1789991 235.2293
ILMN_1702764 122.0698
ILMN_1757106 688.7718
ILMN_1717337 141.8977
ILMN_1677318 259.8267
ILMN_1812607 230.757
ILMN_1764276 174.0958
ILMN_1740252 595.6125
ILMN_1676737 242.5754
ILMN_1747665 107.6657
ILMN_1746673 1201.908
ILMN_1659953 652.369
ILMN_1778079 195.4067
ILMN_1776157 7954.215
ILMN_1763444 166.7228
ILMN_1674893 142.3612

Total number of rows: 22184

Table truncated, full table size 474 Kbytes.




Supplementary data files not provided
Processed data not provided for this record

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