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Sample GSM3553942 Query DataSets for GSM3553942
Status Public on Jul 26, 2019
Title 73
Sample type RNA
Source name TRIM33 inflammatory blood monocytes
Organism Mus musculus
Characteristics genotype/variation: TRIM33
strain: C57BL/6 LyzCre
age: 6-8 weeks
Treatment protocol CD4 T cells from peripheral and mesenteric lymph nodes of C57BL/6 Foxp3-GFP mice were purified by using Dynabeads Untouched Mouse CD4 Cells Kit (Invitrogen). Then, CD4 Treg and Tmem cells were flow-cytometry sorted as CD4+ CD8α- TCRβ+ GFP+ for Treg and CD4+ CD8α- TCRβ+ GFP- CD44hi for Tmem using a FACS-ARIA3 flow cytometer.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using the RNeasy Mini kit (QIAGEN) according to manufacturer's manual.
Label biotin
Label protocol Biotinylated cDNA were prepared according to the standard Nugen Ovation PicoV2 protocol from 900 pg total RNA.
Hybridization protocol Following fragmentation, 3,6 ug of cDNA were hybridized for 17 hr at 45C on GeneChip MouseGene2.0ST Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using Affymetrix GC3000 Scanner.
Description total RNA
Data processing Data were normalized using RMA and statistical analysis was realized on Partek Genomics Suite. CDF file V18 was used for annotation.
Submission date Jan 08, 2019
Last update date Jul 26, 2019
Contact name Franck Letourneur
Organization name INSERM
Department U1016
Lab genomic
Street address 22 rue mechain
City Paris
ZIP/Postal code 75014
Country France
Platform ID GPL18802
Series (1)
GSE124778 TRIM33 deficiency in mature myeloid cells impairs resolution of colonic inflammation

Data table header descriptions
VALUE RMA log2 signal intensity

Data table
74399_at 2.963257638
214403_at 2.693944944
668379_at 2.231498231
387252_at 1.729913522
259027_at 1.806102556
637896_at 1.840919789
237412_at 2.159347244
112422_at 1.562110248
626970_at 1.903756703
692164_at 1.525714178
171183_at 1.98629698
52673_at 2.551367386
258390_at 1.79129244
383548_at 1.737648555
654455_at 1.898229589
692182_at 2.090113125
100040743_at 1.741665091
669149_at 1.908124031
387162_at 1.648419867
385120_at 1.562110248

Total number of rows: 24479

Table truncated, full table size 515 Kbytes.

Supplementary file Size Download File type/resource
GSM3553942_73_MoGene-2_0-st_.CEL.gz 8.0 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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