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Sample GSM3553944 Query DataSets for GSM3553944
Status Public on Jul 26, 2019
Title CTL1
Sample type RNA
Source name WT inflammatory blood monocytes
Organism Mus musculus
Characteristics genotype/variation: WT
strain: C57BL/6 LyzCre
age: 6-8 weeks
Treatment protocol CD4 T cells from peripheral and mesenteric lymph nodes of C57BL/6 Foxp3-GFP mice were purified by using Dynabeads Untouched Mouse CD4 Cells Kit (Invitrogen). Then, CD4 Treg and Tmem cells were flow-cytometry sorted as CD4+ CD8α- TCRβ+ GFP+ for Treg and CD4+ CD8α- TCRβ+ GFP- CD44hi for Tmem using a FACS-ARIA3 flow cytometer.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using the RNeasy Mini kit (QIAGEN) according to manufacturer's manual.
Label biotin
Label protocol Biotinylated cDNA were prepared according to the standard Nugen Ovation PicoV2 protocol from 900 pg total RNA.
Hybridization protocol Following fragmentation, 3,6 ug of cDNA were hybridized for 17 hr at 45C on GeneChip MouseGene2.0ST Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using Affymetrix GC3000 Scanner.
Description total RNA
Data processing Data were normalized using RMA and statistical analysis was realized on Partek Genomics Suite. CDF file V18 was used for annotation.
Submission date Jan 08, 2019
Last update date Jul 26, 2019
Contact name Franck Letourneur
Organization name INSERM
Department U1016
Lab genomic
Street address 22 rue mechain
City Paris
ZIP/Postal code 75014
Country France
Platform ID GPL18802
Series (1)
GSE124778 TRIM33 deficiency in mature myeloid cells impairs resolution of colonic inflammation

Data table header descriptions
VALUE RMA log2 signal intensity

Data table
74399_at 1.501595748
214403_at 1.777487457
668379_at 1.66810063
387252_at 2.558745047
259027_at 2.045765773
637896_at 1.995036706
237412_at 2.496631057
112422_at 1.631677085
626970_at 1.849976641
692164_at 1.646465894
171183_at 1.898220474
52673_at 2.375563969
258390_at 1.967055491
383548_at 1.706107294
654455_at 1.805177941
692182_at 1.921899429
100040743_at 1.785922006
669149_at 1.592041563
387162_at 1.724330488
385120_at 1.365930654

Total number of rows: 24479

Table truncated, full table size 515 Kbytes.

Supplementary file Size Download File type/resource
GSM3553944_CTL1_MoGene-2_0-st_.CEL.gz 8.1 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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