|
Status |
Public on Jul 26, 2019 |
Title |
CTL2 |
Sample type |
RNA |
|
|
Source name |
WT inflammatory blood monocytes
|
Organism |
Mus musculus |
Characteristics |
genotype/variation: WT strain: C57BL/6 LyzCre age: 6-8 weeks
|
Treatment protocol |
CD4 T cells from peripheral and mesenteric lymph nodes of C57BL/6 Foxp3-GFP mice were purified by using Dynabeads Untouched Mouse CD4 Cells Kit (Invitrogen). Then, CD4 Treg and Tmem cells were flow-cytometry sorted as CD4+ CD8α- TCRβ+ GFP+ for Treg and CD4+ CD8α- TCRβ+ GFP- CD44hi for Tmem using a FACS-ARIA3 flow cytometer.
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was extracted using the RNeasy Mini kit (QIAGEN) according to manufacturer's manual.
|
Label |
biotin
|
Label protocol |
Biotinylated cDNA were prepared according to the standard Nugen Ovation PicoV2 protocol from 900 pg total RNA.
|
|
|
Hybridization protocol |
Following fragmentation, 3,6 ug of cDNA were hybridized for 17 hr at 45C on GeneChip MouseGene2.0ST Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
Scan protocol |
GeneChips were scanned using Affymetrix GC3000 Scanner.
|
Description |
total RNA
|
Data processing |
Data were normalized using RMA and statistical analysis was realized on Partek Genomics Suite. CDF file V18 was used for annotation.
|
|
|
Submission date |
Jan 08, 2019 |
Last update date |
Jul 26, 2019 |
Contact name |
Franck Letourneur |
E-mail(s) |
franck.letourneur@inserm.fr
|
Organization name |
INSERM
|
Department |
U1016
|
Lab |
genomic
|
Street address |
22 rue mechain
|
City |
Paris |
ZIP/Postal code |
75014 |
Country |
France |
|
|
Platform ID |
GPL18802 |
Series (1) |
GSE124778 |
TRIM33 deficiency in mature myeloid cells impairs resolution of colonic inflammation |
|