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Sample GSM3553945 Query DataSets for GSM3553945
Status Public on Jul 26, 2019
Title CTL2
Sample type RNA
Source name WT inflammatory blood monocytes
Organism Mus musculus
Characteristics genotype/variation: WT
strain: C57BL/6 LyzCre
age: 6-8 weeks
Treatment protocol CD4 T cells from peripheral and mesenteric lymph nodes of C57BL/6 Foxp3-GFP mice were purified by using Dynabeads Untouched Mouse CD4 Cells Kit (Invitrogen). Then, CD4 Treg and Tmem cells were flow-cytometry sorted as CD4+ CD8α- TCRβ+ GFP+ for Treg and CD4+ CD8α- TCRβ+ GFP- CD44hi for Tmem using a FACS-ARIA3 flow cytometer.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using the RNeasy Mini kit (QIAGEN) according to manufacturer's manual.
Label biotin
Label protocol Biotinylated cDNA were prepared according to the standard Nugen Ovation PicoV2 protocol from 900 pg total RNA.
Hybridization protocol Following fragmentation, 3,6 ug of cDNA were hybridized for 17 hr at 45C on GeneChip MouseGene2.0ST Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using Affymetrix GC3000 Scanner.
Description total RNA
Data processing Data were normalized using RMA and statistical analysis was realized on Partek Genomics Suite. CDF file V18 was used for annotation.
Submission date Jan 08, 2019
Last update date Jul 26, 2019
Contact name Franck Letourneur
Organization name INSERM
Department U1016
Lab genomic
Street address 22 rue mechain
City Paris
ZIP/Postal code 75014
Country France
Platform ID GPL18802
Series (1)
GSE124778 TRIM33 deficiency in mature myeloid cells impairs resolution of colonic inflammation

Data table header descriptions
VALUE RMA log2 signal intensity

Data table
74399_at 3.369223634
214403_at 1.806829114
668379_at 1.697131003
387252_at 1.766654269
259027_at 1.799383336
637896_at 2.350192551
237412_at 1.906165382
112422_at 1.841953995
626970_at 1.771543445
692164_at 1.923443847
171183_at 1.804723003
52673_at 1.737066805
258390_at 1.997410763
383548_at 1.761449678
654455_at 1.938848091
692182_at 1.758602265
100040743_at 2.131934104
669149_at 2.722769223
387162_at 1.747564628
385120_at 1.766951544

Total number of rows: 24479

Table truncated, full table size 515 Kbytes.

Supplementary file Size Download File type/resource
GSM3553945_CTL2_MoGene-2_0-st_.CEL.gz 8.0 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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