|
| Status |
Public on Jul 26, 2019 |
| Title |
CTL9 |
| Sample type |
RNA |
| |
|
| Source name |
WT inflammatory blood monocytes
|
| Organism |
Mus musculus |
| Characteristics |
genotype/variation: WT
strain: C57BL/6 LyzCre
age: 6-8 weeks
|
| Treatment protocol |
CD4 T cells from peripheral and mesenteric lymph nodes of C57BL/6 Foxp3-GFP mice were purified by using Dynabeads Untouched Mouse CD4 Cells Kit (Invitrogen). Then, CD4 Treg and Tmem cells were flow-cytometry sorted as CD4+ CD8α- TCRβ+ GFP+ for Treg and CD4+ CD8α- TCRβ+ GFP- CD44hi for Tmem using a FACS-ARIA3 flow cytometer.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using the RNeasy Mini kit (QIAGEN) according to manufacturer's manual.
|
| Label |
biotin
|
| Label protocol |
Biotinylated cDNA were prepared according to the standard Nugen Ovation PicoV2 protocol from 900 pg total RNA.
|
| |
|
| Hybridization protocol |
Following fragmentation, 3,6 ug of cDNA were hybridized for 17 hr at 45C on GeneChip MouseGene2.0ST Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using Affymetrix GC3000 Scanner.
|
| Description |
total RNA
|
| Data processing |
Data were normalized using RMA and statistical analysis was realized on Partek Genomics Suite. CDF file V18 was used for annotation.
|
| |
|
| Submission date |
Jan 08, 2019 |
| Last update date |
Jul 26, 2019 |
| Contact name |
Franck Letourneur |
| E-mail(s) |
franck.letourneur@inserm.fr
|
| Organization name |
INSERM
|
| Department |
U1016
|
| Lab |
genomic
|
| Street address |
22 rue mechain
|
| City |
Paris |
| ZIP/Postal code |
75014 |
| Country |
France |
| |
|
| Platform ID |
GPL18802 |
| Series (1) |
| GSE124778 |
TRIM33 deficiency in mature myeloid cells impairs resolution of colonic inflammation |
|
