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Sample GSM3553948 Query DataSets for GSM3553948
Status Public on Jul 26, 2019
Title CTL_mix_1
Sample type RNA
 
Source name WT inflammatory blood monocytes
Organism Mus musculus
Characteristics genotype/variation: WT
strain: C57BL/6 LyzCre
age: 6-8 weeks
Treatment protocol CD4 T cells from peripheral and mesenteric lymph nodes of C57BL/6 Foxp3-GFP mice were purified by using Dynabeads Untouched Mouse CD4 Cells Kit (Invitrogen). Then, CD4 Treg and Tmem cells were flow-cytometry sorted as CD4+ CD8α- TCRβ+ GFP+ for Treg and CD4+ CD8α- TCRβ+ GFP- CD44hi for Tmem using a FACS-ARIA3 flow cytometer.
Extracted molecule total RNA
Extraction protocol Total RNA was extracted using the RNeasy Mini kit (QIAGEN) according to manufacturer's manual.
Label biotin
Label protocol Biotinylated cDNA were prepared according to the standard Nugen Ovation PicoV2 protocol from 900 pg total RNA.
 
Hybridization protocol Following fragmentation, 3,6 ug of cDNA were hybridized for 17 hr at 45C on GeneChip MouseGene2.0ST Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
Scan protocol GeneChips were scanned using Affymetrix GC3000 Scanner.
Description total RNA
Data processing Data were normalized using RMA and statistical analysis was realized on Partek Genomics Suite. CDF file V18 was used for annotation.
 
Submission date Jan 08, 2019
Last update date Jul 26, 2019
Contact name Franck Letourneur
E-mail(s) franck.letourneur@inserm.fr
Organization name INSERM
Department U1016
Lab genomic
Street address 22 rue mechain
City Paris
ZIP/Postal code 75014
Country France
 
Platform ID GPL18802
Series (1)
GSE124778 TRIM33 deficiency in mature myeloid cells impairs resolution of colonic inflammation

Data table header descriptions
ID_REF
VALUE RMA log2 signal intensity

Data table
ID_REF VALUE
74399_at 1.869647643
214403_at 1.709676936
668379_at 1.656347962
387252_at 1.992648911
259027_at 1.634517623
637896_at 2.547923163
237412_at 1.419842777
112422_at 1.57607633
626970_at 1.78159905
692164_at 1.630546778
171183_at 1.642618498
52673_at 2.11317637
258390_at 1.868088141
383548_at 1.637552817
654455_at 1.849179918
692182_at 1.896914428
100040743_at 1.705977229
669149_at 1.911551097
387162_at 1.685419233
385120_at 1.501514974

Total number of rows: 24479

Table truncated, full table size 515 Kbytes.




Supplementary file Size Download File type/resource
GSM3553948_CTL_mix_1_MoGene-2_0-st_.CEL.gz 8.2 Mb (ftp)(http) CEL
Raw data provided as supplementary file
Processed data included within Sample table

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