|
| Status |
Public on Mar 08, 2010 |
| Title |
dnPI3KSham_417 |
| Sample type |
RNA |
| |
|
| Source name |
dnPI3K Sham
|
| Organism |
Mus musculus |
| Characteristics |
strain C57BL/6
Left ventricle
|
| Treatment protocol |
As specified above for 6 mouse models of increasing cardiac stress.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Snap frozen heart samples in liquid nitrogen. TRIzol extraction of total RNA. Quality and integrity of small RNA assayed using Agilent Bioanalyzer.
|
| Label |
Cy3
|
| Label protocol |
Refer to Agilent miRNA labeling protocol
|
| |
|
| Hybridization protocol |
Refer to Agilent miRNA hybridisation protocol
|
| Scan protocol |
Feature extraction 10.1 used
|
| Description |
Agilent one-colour 8X15K miRNA microarray
|
| Data processing |
Data were processed using alogorithms specified by the Agilent Feature Extraction software. Subsequent analysis of data were carried out using both Partek v6 and GeneSpring v10.
|
| |
|
| Submission date |
Jan 02, 2009 |
| Last update date |
Mar 04, 2010 |
| Contact name |
Ruby CY Lin |
| E-mail(s) |
rubyl@unsw.edu.au, ruby.lin@sydney.edu.au
|
| Organization name |
The Westmead Institute for Medical Research
|
| Street address |
176 Hawkesbury Road, Westmead
|
| City |
Sydney |
| State/province |
NSW |
| ZIP/Postal code |
2145 |
| Country |
Australia |
| |
|
| Platform ID |
GPL8824 |
| Series (1) |
| GSE14267 |
microRNA profiling of myocardial infarction on a PI3K setting in mouse models |
|
