|
| Status |
Public on Nov 30, 2019 |
| Title |
exp4_07: HT29_3D_CRLM |
| Sample type |
RNA |
| |
|
| Source name |
HT-29 cells
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: HT29
cell type: human colon adenocarcinoma cell line
type of culture: 3D_CRLM
cell type: Decellularized matrix from Patient4
|
| Growth protocol |
The human colon adenocarcinoma cell line HT29 ZsGreen/Luc+ was maintained in RPMI-1640 medium supplemented with 10 %fetal bovine serum, 1 % L-Glutamine and 1 % pen/strep antibiotic solution (growth medium) in humidified atmosphere at 37 °C in 5 % CO2. HC, CRC, HL and CRLM decellularized matrices were then injected with 2.5x105 HT-29 ZsGreen/Luc+ cells, resuspended in 10μL of Collagen I (diluted 2:3 with RPMI-1640). Complete medium was carefully added and changed every day
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted from cells, HL and CRLM recellularized matrices using TissueLyser (Qiagen) combined with Trizol in 3 independent experiments
|
| Label |
biotin
|
| Label protocol |
Labeled and Fragmented ss-cDNA were prepared according to the standard GeneChip WT Plus protocol starting from 100 ng of Total RNA
|
| |
|
| Hybridization protocol |
Following fragmentation and labelling,5.5 μg ss-cDNA were hybridized for 16 hr at 45C on GeneChip Human Genome Clariom S Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the Scan GCS3000.
|
| Description |
Gene expression data from HT29 cell growth in 3D with a decellularized matrix derived form a CRLM
|
| Data processing |
The data were normalized using R software [www.r-project.org] with BioConductor package [www.bioconductor.org] using RMA
|
| |
|
| Submission date |
Jan 21, 2019 |
| Last update date |
Dec 01, 2019 |
| Contact name |
silvia bresolin |
| E-mail(s) |
silvia.bresolin@unipd.it
|
| Phone |
+390498215487
|
| Organization name |
università di padova
|
| Department |
department of women's and children's health
|
| Lab |
SSD ematologia-clinica sperimentale
|
| Street address |
via giustiniani 3
|
| City |
padova |
| State/province |
padova |
| ZIP/Postal code |
35128 |
| Country |
Italy |
| |
|
| Platform ID |
GPL23159 |
| Series (1) |
| GSE125404 |
Patient-derived scaffolds of colorectal cancer metastases as an organotypic 3D model of liver metastatic colonization |
|
