NCBI Logo
GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
          Go
Sample GSM357435 Query DataSets for GSM357435
Status Public on Jan 04, 2010
Title WT_3_no_treatment
Sample type RNA
 
Source name WT_no_serum_treatment
Organism Mus musculus
Characteristics cultured myocytes
Biomaterial provider The University of Washington (Seattle, WA).
Treatment protocol Transgenic mice overexpressing CHF1/Hey2 in the myocardium under the control of the mlc2v promoter and neonatal mouse cardiac myocyte culture was performed as previously described. To induce hypertrophy, neonatal myocytes in serum free medium were exposed to media with 20% fetal bovine serum for 24 hours.
Extracted molecule total RNA
Extraction protocol RNA was isolated from cultured myocytes by extraction with a commercially available kit (Qiagen) according to the manufacturer’s instructions.
Label biotin
Label protocol For microarray hybridization, RNA was processed for hybridization to Affymetrix GeneChip® Mouse Gene 1.0 ST Arrays through a core facility in the Functional Genomics Laboratory at the Center for Ecogenetics and Environmental Health at the University of Washington according to the manufacturer’s instructions.
 
Hybridization protocol The standard hybridization protocol was used as recommended by Affymterix.
Scan protocol GeneChips were scanned using the Affymetrix GeneArray Scanner 3000 through the University of Washington Functional Genomics Laboratory at the Center for Ecogenetics and Environmental Health (http://depts.washington.edu/ceeh/ServiceCores/FC1/FC1.html).
Description cultured myocytes.
Data processing Affymetrix Expression Console v1.1 RMA normalization log2
 
Submission date Jan 05, 2009
Last update date Sep 05, 2017
Contact name James William MacDonald
E-mail(s) jmacdon@uw.edu
Organization name University of Washington
Department Environmental and Occupational Health Sciences
Street address 4225 Roosevelt Way NE
City Seattle
State/province WA
ZIP/Postal code 98105-6099
Country USA
 
Platform ID GPL6246
Series (1)
GSE14288 Transcription Factor CHF1/Hey2 Regulates Specific Gene Sets in Serum Stimulated Primary Cardiac Myocytes

Data table header descriptions
ID_REF
VALUE RMA normalized signal log2

Data table
ID_REF VALUE
10344614 5.379307
10344616 2.977201
10344620 3.358857
10344622 5.680822
10344624 9.029789
10344633 9.565267
10344637 8.347194
10344653 3.318836
10344658 6.955673
10344674 3.42769
10344679 4.816898
10344707 7.486059
10344713 9.466534
10344715 3.585677
10344717 4.070468
10344719 5.750861
10344721 3.018705
10344723 6.053193
10344725 4.500245
10344741 9.688304

Total number of rows: 35557

Table truncated, full table size 621 Kbytes.




Supplementary file Size Download File type/resource
GSM357435.CEL.gz 4.1 Mb (ftp)(http) CEL
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap