|
| Status |
Public on May 27, 2009 |
| Title |
Testis_M125 |
| Sample type |
RNA |
| |
|
| Source name |
irradiated mouse testis_0.65 μGy/min (420 mGy)
|
| Organism |
Mus musculus |
| Characteristics |
Male specific pathogen free C57BL/6J
|
| Biomaterial provider |
CLEA Japan, Inc.
|
| Treatment protocol |
Irradiation with 137Cs γ-rays was carried out in a facility at the Institute for Environmental Sciences for 485 days (22 h/day) at dose-rates of 0.032 μGy/min, 0.65 μGy/min, and 13 μGy/min. The total dose after irradiation at these dose-rates was 21 mGy, 420 mGy, and 8000 mGy. We examined 12 mice (3 per each dose-rate condition, including the control). Mice were euthanized immediately after the termination of irradiation, and the kidney and testis were removed and stored at -80°C in RNAlater (Ambion).
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNAlater and RNeasy Mini Kit and RNase-Free DNase Set (Qiagen, Valencia, CA)
|
| Label |
biotin
|
| Label protocol |
Illumina RNA Amplification Kit (Label with biotin-UTP directly)
|
| |
|
| Hybridization protocol |
Illumina Gene Expression system Hyb E1 and Block Buffer kit,
Wash E1BC Buffer kit and High Temp Wash Buffer kit
|
| Scan protocol |
Standard of BeadStation 500GX-WG system (Illumina)
|
| Description |
Sample identifier: M07 M125
|
| Data processing |
Rank-invariant normalization (http://www.mged.org/normalization)
|
| |
|
| Submission date |
Jan 06, 2009 |
| Last update date |
May 27, 2009 |
| Contact name |
Mitsuru Nenoi |
| Fax |
81-43-255-6497
|
| URL |
http://www.nirs.go.jp/ENG/research/radiation_protection/05.shtml
|
| Organization name |
National institute of Radiological Sciences
|
| Department |
Research Center for Radiation Protection
|
| Lab |
Radiation Effect Mechanisms Research Group
|
| Street address |
4-9-1, Anagawa, Inage-ku
|
| City |
Chiba |
| ZIP/Postal code |
263-8555 |
| Country |
Japan |
| |
|
| Platform ID |
GPL4234 |
| Series (1) |
| GSE14290 |
Microarray analysis of gene expression in the kidneys and testes of mice after low-dose-rate irradiation |
|
