|
| Status |
Public on Jan 24, 2019 |
| Title |
EC-IL1b-VC, biological replicate-1 |
| Sample type |
RNA |
| |
|
| Source name |
HPAECs-IL1b-vehicle control
|
| Organism |
Homo sapiens |
| Characteristics |
tissue: Human pulmonary artery endothelial cells
|
| Treatment protocol |
The cells were treated with IL-1b (10ng/ml) either with vechicle control (DMSO) or I-BET762 (500nM) for 48 h in complete media.
|
| Growth protocol |
HPAECs were cultured in endothelial cell growth medium from Lonza. All the experiments were done in the cells with passage number 4-8.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was isolated using Qiazol extraction method from Qiagen according to manufacturer's instructions.
|
| Label |
biotin
|
| Label protocol |
Biotiny end labeled ss-cDNA were prepared according to Affymetrix WT pico protocol from 4 ng total RNA (GeneChip WT pico Reagent kit P/N 703262 rev 4).
|
| |
|
| Hybridization protocol |
Following fragmentation and end label, 2.4 ug of ss-cDNA were hybridized for 16 hr at 45C on GeneChip Human Clariom S. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using the GeneChip Scanner with 7G upgrade.
|
| Description |
Time of treatments - 48h
|
| Data processing |
The data were analyzed with Gene Expression Console using Gene level SST-RMA with default settings.
|
| |
|
| Submission date |
Jan 23, 2019 |
| Last update date |
Jan 24, 2019 |
| Contact name |
Vijay Yechoor |
| E-mail(s) |
yechoorv@pitt.edu
|
| Organization name |
University of Pittsburgh
|
| Department |
Vascular Medicine Institute
|
| Street address |
200 Lothrop street,
|
| City |
Pittsburgh |
| State/province |
PA |
| ZIP/Postal code |
15261 |
| Country |
USA |
| |
|
| Platform ID |
GPL23159 |
| Series (1) |
| GSE125508 |
Gene expression profiling of IL1b induced primary human pulmonary artery endothelial cells (HPAECs) treated with or without I-BET762 |
|
