|
| Status |
Public on Aug 23, 2019 |
| Title |
RMG1-siCPNE8 #A |
| Sample type |
RNA |
| |
|
| Source name |
RMG1 cells treated with siCPNE8 #A
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: RMG1
cell type: ovarian cancer cell line
genotype/variation: siCPNE8
|
| Treatment protocol |
RMG1 cells were transfected with CPNE8-targeting siRNAs or control siRNA, and OVCAR3 cells were transfected with BHLHE41-targeting siRNAs or control siRNA.
|
| Growth protocol |
Human ovarian cancer RMG1 and OVCAR3 cell lines were maintained in Dulbecco's modified Eagle's medium (DMEM) and Roswell Park Memorial Institute (RPMI) 1640 medium, respectively.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted using ISOGEN reagent (Nippon Gene, Japan).
|
| Label |
biotin
|
| Label protocol |
Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 150 ng total RNA.
|
| |
|
| Hybridization protocol |
Following fragmentation, 4 ug of cRNA were hybridized for 16 hr at 45C on Affymetrix GeneChip (Clario S Assay, human). GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
The samples were scanned on a GeneChip Scanner 3000 7G (Affymetrix).
|
| Description |
Gene expression data from RMG1 cells treated with siRNA targeting CPNE8 (siCPNB8 #A)
|
| Data processing |
The microarray data were preprocessed with Affymetrix Expression Console software (build.1.2.1.20) using Affymetrix default Robust Multichip Analysis (RMA) sketch algorithm workflow.
|
| |
|
| Submission date |
Jan 23, 2019 |
| Last update date |
Aug 24, 2019 |
| Contact name |
Kazuhiro Ikeda |
| Organization name |
Saitama Medical University
|
| Street address |
1397-1 Yamane
|
| City |
Hidaka |
| State/province |
Saitama |
| ZIP/Postal code |
350-1241 |
| Country |
Japan |
| |
|
| Platform ID |
GPL23159 |
| Series (1) |
| GSE125542 |
Gene expression data from human ovarian cancer cells treated with siRNAs targeting CPNE8 and BHLHE41 |
|
