|
| Status |
Public on Apr 18, 2019 |
| Title |
M- DMAD embryos(3h) (replicate 2) |
| Sample type |
SRA |
| |
|
| Source name |
3h M- DMAD rep 2
|
| Organism |
Drosophila melanogaster |
| Characteristics |
strain: M- DMAD
tissue: embryo
age: 3h
|
| Extracted molecule |
polyA RNA |
| Extraction protocol |
Total RNAs were isolated from embryos at indicated stages using TRIzol reagent (Invitrogen) and mRNAs were then purified using NEBNext® Poly(A) mRNA Magnetic Isolation Module according to the user manual.
Illumina sequencing libraries were constructed according to the manufacturer’s instructions. The libraries were then sequenced using the Illumina Hiseq2500 V4 and NovaSeq platform
|
| |
|
| Library strategy |
RNA-Seq |
| Library source |
transcriptomic |
| Library selection |
cDNA |
| Instrument model |
Illumina NovaSeq 6000 |
| |
|
| Description |
3h_DMADMut_FPKM.txt
|
| Data processing |
FastQC was used to make sure that the sequencing data were high quality
Reads were aligned to the dm6 genome assembly using Tophat2/STAR
Differential expression analysis was carried out by cuffdiff
Genome_build: dm6
|
| |
|
| Submission date |
Jan 23, 2019 |
| Last update date |
Apr 18, 2019 |
| Contact name |
Shunmin He |
| E-mail(s) |
hesm@ioz.ac.cn
|
| Phone |
010-64807279
|
| Organization name |
Institute of Zoology, Chinese Academy of Sciences
|
| Street address |
1 Beichen West Road, Chaoyang District
|
| City |
Beijing |
| ZIP/Postal code |
100101 |
| Country |
China |
| |
|
| Platform ID |
GPL25244 |
| Series (1) |
| GSE86795 |
6mA-DNA-binding factor Jumu controls maternal-to-zygotic transition upstream of Zelda |
|
| Relations |
| BioSample |
SAMN10796123 |
| SRA |
SRX5284315 |
