|
| Status |
Public on Jan 25, 2019 |
| Title |
Cancer4-3 |
| Sample type |
RNA |
| |
|
| Source name |
sebaceous gland carcinoma of the eyelid
|
| Organism |
Homo sapiens |
| Characteristics |
strain: female
age: 58 years
tissue: eyelid
diagnosis: sebaceous gland carcinoma of the eyelid
|
| Treatment protocol |
Sebaceous gland carcinoma and sebaceous adenoma samples were used.
|
| Growth protocol |
We enrolled three patients (74-, and 83- and 58-year-old women) with human sebaceous gland carcinoma of the eyelid and one patient (92-year-old man) with human sebaceouos adenoma of the eyelid. Four patients underwent surgical excision of the tumor.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted from cells using a NucleoSpin® miRNA isolation kit (MACHEREY-NAGEL GmbH) along with on-column DNase I treatment. RNA quality was analyzed using a Bioanalyzer 2100 (Agilent Technologies). RNA samples with RIN (RNA integrity number) values
|
| Label |
biotin
|
| Label protocol |
500 ng of total RNA was used to synthesize cRNA with a GeneChip WT PLUS Reagent Kit (Affymetrix).
|
| |
|
| Hybridization protocol |
After fragmentation, the biotinylated cRNA was hybridized to arrays at 45°C for 16 h. The arrays were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
The arrays were scanned by the Affymetrix Gene ChIP Scanner 3000 7G
|
| Description |
Patient 3, sebaceous gland carcinoma of the eyelid
|
| Data processing |
The data were analyzed using Gene Spring software with RMA normalization method.
|
| |
|
| Submission date |
Jan 24, 2019 |
| Last update date |
Jan 25, 2019 |
| Contact name |
Tetsushi Hirano |
| E-mail(s) |
thirano@cts.u-toyama.ac.jp
|
| Organization name |
University of Toyama
|
| Department |
Life Science Research Center
|
| Street address |
2630 sugitani
|
| City |
Toyama |
| State/province |
Toyama |
| ZIP/Postal code |
939 0194 |
| Country |
Japan |
| |
|
| Platform ID |
GPL23159 |
| Series (1) |
| GSE125582 |
Identification of genes involved in sebaceous gland carcinoma of the eyelid |
|
