|
| Status |
Public on Jan 28, 2021 |
| Title |
MDA-MB-231 shDROSHA, biological rep1 |
| Sample type |
RNA |
| |
|
| Source name |
MDA-MB-231
|
| Organism |
Homo sapiens |
| Characteristics |
cell line: MDA-MB-231
transfection: shDROSHA
|
| Treatment protocol |
not applicable
|
| Growth protocol |
MDA-MB-231 cells were cultured in Leibovitz's L-15 Medium supplemented with 10% FBS and penicillin/streptomycin.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Total RNA was extracted by Trizol/Chloroform, and then purified with NucleoSpin® miRNA (Cat No 740971.250,Macherey-Nagel).cDNA synthesis and amplification was performed using the WT cDNA synthesis and amplification kit (Affymetrix).
|
| Label |
biotin
|
| Label protocol |
Biotinylated ss-cDNA were prepared according to the standard Affymetrix protocol from 500 ng total RNA (GeneChipTM WT PLUS Reagent Kit, 2017, Affymetrix)
|
| |
|
| Hybridization protocol |
Following Fragment and Label Single-Stranded cDNA, 27ul of biotin-labeled ss-cDNA sample were hybridized for 16 hr at 45℃ on GeneChip Clariom S Human Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 450.
|
| Scan protocol |
GeneChips were scanned using GeneChip Scanner 3000 7G.
|
| Data processing |
The data were analyzed with robust multi-array average (RMA) using Affymetrix default analysis settings and global scaling as normalization method.
|
| |
|
| Submission date |
Jan 27, 2019 |
| Last update date |
Jan 28, 2021 |
| Contact name |
jinxin lu |
| E-mail(s) |
lujinxin0321@gmail.com
|
| Phone |
18304019315
|
| Organization name |
Dalian Medical University
|
| Street address |
lvshunkou district lvshun south goad west sector No.9
|
| City |
Dalian |
| State/province |
Liaoning |
| ZIP/Postal code |
116044 |
| Country |
China |
| |
|
| Platform ID |
GPL23159 |
| Series (1) |
| GSE125705 |
Identification of genes are regulated by DROSHA at mRNA level [microarray] |
|
