|
| Status |
Public on Feb 06, 2019 |
| Title |
CTD1uM-24h-1 |
| Sample type |
RNA |
| |
|
| Source name |
human neuroblastoma
|
| Organism |
Homo sapiens |
| Characteristics |
strain: female
age: 4 years
tissue: bone marrow
cell line: SH-SY5Y cells (ECACC, No. 94030304)
treatment: CTD1uM-24h
|
| Treatment protocol |
Semi-confluent cells were exposued to 1uM of CTD or vehicle (0.1%DMSO) dossolved in medium for 24 hours.
|
| Growth protocol |
Human neuroblastoma SH-SY5Y cells (ECACC, No. 94030304) were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) with 10% fetal bovine serum (FBS), 100 U/ml penicillin and 100 µg/ml streptomycin at 37 °C in humidified air with 5% CO2.
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA was extracted from cells using a NucleoSpin® RNA isolation kit (MACHEREY-NAGEL GmbH) along with on-column DNase I treatment. RNA quality was analyzed using a Bioanalyzer 2100 (Agilent Technologies). RNA samples with RIN (RNA integrity number) values >5.0 were considered acceptable.
|
| Label |
biotin
|
| Label protocol |
500 ng of total RNA was used to synthesize cRNA with a GeneChip WT PLUS Reagent Kit (Affymetrix).
|
| |
|
| Hybridization protocol |
After fragmentation, the biotinylated cRNA was hybridized to arrays at 45°C for 16 h.
|
| Scan protocol |
The arrays were washed and stained in the Affymetrix Fluidics Station 450.
|
| Data processing |
The data were analyzed with RMA using Affymetrix default analysis settings and global scaling as normalization method.
|
| |
|
| Submission date |
Feb 05, 2019 |
| Last update date |
Feb 06, 2019 |
| Contact name |
Tetsushi Hirano |
| E-mail(s) |
thirano@cts.u-toyama.ac.jp
|
| Organization name |
University of Toyama
|
| Department |
Life Science Research Center
|
| Street address |
2630 sugitani
|
| City |
Toyama |
| State/province |
Toyama |
| ZIP/Postal code |
939 0194 |
| Country |
Japan |
| |
|
| Platform ID |
GPL23159 |
| Series (1) |
| GSE126103 |
Stimulative effect of low-dose neonicotinoid pesticide, clothianidin on human neuroblastoma SH-SY5Y cells by disrupting intracellular signaling |
|
