|
Status |
Public on Oct 01, 2009 |
Title |
mouse mast cells, replicate 1 |
Sample type |
RNA |
|
|
Channel 1 |
Source name |
Mouse in vitro-differentiated mast cells, treated by unspecific goat IgG (control), replicate 1
|
Organism |
Mus musculus |
Characteristics |
Mouse in vitro-differentiated mast cells, treated by unspecific goat IgG (control)
|
Treatment protocol |
control goat IgG, 15 ug/ml, 2 hours, in vitro
|
Growth protocol |
Bone marrow cells were differentiated in RPMI + 10% FCS + 5 ng/ml mouse IL-3 + 40 ng/ml mouse SCF for >4 weeks.
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was extracted by Qiagen RNeasy Micro Kit. The quality and quantity of the RNA was checked by Agilent Bioanalyzer and Nanodrop, respectively.
|
Label |
Cy3
|
Label protocol |
Agilent Dual-Color Gene Expression Protocol version 5.5
|
|
|
Channel 2 |
Source name |
Mouse in vitro-differentiated mast cells, treated by an agonist anti-mouse TIM-3 antibody, replicate 1
|
Organism |
Mus musculus |
Characteristics |
Mouse in vitro-differentiated mast cells, treated by an agonist anti-mouse TIM-3 antibody (RnD Systems)
|
Treatment protocol |
agonist polyclonal goat anti-mouse TIM3 antibody (RnD Systems), 15 ug/ml, 2 hours, in vitro
|
Growth protocol |
Bone marrow cells were differentiated in RPMI + 10% FCS + 5 ng/ml mouse IL-3 + 40 ng/ml mouse SCF for >4 weeks.
|
Extracted molecule |
total RNA |
Extraction protocol |
RNA was extracted by Qiagen RNeasy Micro Kit. The quality and quantity of the RNA was checked by Agilent Bioanalyzer and Nanodrop, respectively.
|
Label |
Cy5
|
Label protocol |
Agilent Dual-Color Gene Expression Protocol version 5.5
|
|
|
|
Hybridization protocol |
Agilent Dual-Color Gene Expression Protocol version 5.5
|
Scan protocol |
Agilent Scanner, extended dynamic range (high value: 100%, low value: 10%)
|
Description |
Washing according to Agilent Dual-Color Gene Expression Protocol version 5.5
|
Data processing |
Agilent Feature Extraction Software version 9, with default 4x44K settings
|
|
|
Submission date |
Jan 14, 2009 |
Last update date |
Jul 22, 2009 |
Contact name |
Zoltan Wiener |
E-mail(s) |
wiezol@dgci.sote.hu
|
Phone |
+3612102929
|
Fax |
+3613036968
|
URL |
http://www.dgci.sote.hu
|
Organization name |
Semmelweis University
|
Department |
Dept of Genetics, Cell- and Immunobiology
|
Street address |
Nagyvarad ter 4.
|
City |
Budapest |
ZIP/Postal code |
H-1089 |
Country |
Hungary |
|
|
Platform ID |
GPL4134 |
Series (1) |
GSE14438 |
The effect of TIM-3 stimulation on the gene expression profile of IgE/antigen-activated mouse mast cells |
|